Oncostatin M/OSM Antibody

Western Blot: Oncostatin M/OSM Antibody [H00005008-D01] - Analysis of OSM expression in transfected 293T cell line by OSM polyclonal antibody.Lane 1: OSM transfected lysate(28.50 KDa).Lane 2: Non-transfected lysate.

Western Blot: Oncostatin M/OSM Antibody [H00005008-D01] - Analysis of OSM expression in transfected 293T cell line (H00005008-T02) by OSM MaxPab polyclonal antibody. Lane 1: OSM transfected lysate(28.50 KDa). Lane 2: Non-transfected lysate.

Immunoprecipitation: Oncostatin M/OSM Antibody [H00005008-D01] - OSM transfected lysate using anti-OSM MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with OSM purified MaxPab mouse polyclonal antibody (B02P) (H00005008-B02P).

Product Discontinued

Oncostatin M/OSM Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: OSM (NP_065391.1, 1 a.a. - 252 a.a.) full-length human protein. MGVLLTQRTLLSLVLALLFPSMASMAAIGSCSKEYRVLLGQLQKQTDLMQDTSRLLDPYIRIQGLDVPKLREHCRERPGAFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDLEKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQRKLEGCRFLHGYHRFMHSVGRVFSKWGESPNRSRRHSPHQALRKGVRRTRPSRKGKRLMTRGQLPR
• Specificity: OSM - oncostatin M,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OSM
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Experiments in IP: Recommended starting volume is 30-40ul antibody and 10ul protein A magnetic beads (50ug/ul). For over expression lysate with total protein concentration of 1ug/ul, the recommended starting value for loading sample is 200 ul. For other sample types, the amount of lysate will need to be optimised by each end-user

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: No additive
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Oncostatin M/OSM Antibody

• MGC20461
• oncostatin M
• oncostatin-M
• OSM

Background

Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OSM