OMgp Antibody [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

OMgp Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse OMgp (R&D Systems, Catalog # 1674-MG)
  Ile25-Ser420
  Accession # Q63912
• Specificity: Detects mouse OMgp in direct ELISAs and Western blots. In these formats, approximately 35% cross‑reactivity with recombinant human OMgp is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OMgp Antibody [Alexa Fluor® 647]

• oligodendrocyte myelin glycoprotein
• OMG
• OMgp
• OMGPoligodendrocyte-myelin glycoprotein

Background

Oligodendrocyte myelin glycoprotein (OMgp or OMG), Nogo, and myelin-associated glycoprotein (MAG), are three myelin-derived axon outgrowth inhibitors that collapse axonal growth cones and inhibit neurite outgrowth (1 - 3). These three structurally distinct proteins contribute to the myelin-associated inhibitory activity that prevents axonal regeneration after injury of the adult central nervous system (CNS). Mouse OMgp cDNA encodes a 440 amino acid (aa) residue glycosylphosphatidylinositol (GPI)-anchored protein that has a 24 aa signal peptide, eight leucine-rich repeats (LRR) followed by five serine/threonine-rich repeats (4). OMgp has multiple potential N-glycosylation and O-glycosylation sites. Mouse and human OMgp share approximately 88% aa sequence identity. OMgp is expressed on the surface of oligodendrocytes and on large projection neurons, including Purkinje cells of the cerebellum, pyramidal cells of the hippocampus, motoneurons of the brainstem and anterior horn cells of the spinal cord (5). The neurite outgrowth inhibitory activities of all three myelin-derived proteins are mediated by binding to a common receptor complex consisting of the Nogo receptor (NgR) and the p75 neurotrophin receptor (NGFR) (2, 3). Besides its function in the inhibition of axonal growth, OMgp has also been implicated in the inhibition of proliferation. Although the transmembrane receptor that mediates the proliferation inhibition activity has not been identified, the LRR repeats of OMgp were shown to be essential for both the proliferation inhibition and neurite outgrowth inhibition activities (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.