ODF2 [p Ser796] Antibody

Images

 
Western Blot: ODF2 [p Ser796] Antibody [NB110-85525] - Lane 1: Human Semen Lysate. Lane 2: MCF7 WCL. Lane 3: Molt 4 WCL. Lane 4: HeLa WCL. Load: 10ug per lane. Primary Antibody: 1ug/mL blocked with incubated with ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ODF2 [p Ser796] Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near S796 of human cenexin-1.
Modification
p Ser796
Specificity
This is specific for human Cenexin-1 protein phosphorylated at Ser 796.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ODF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:2000
  • ELISA 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Expect a band approximately 93 kDa in size corresponding - phosphorylated Cenexin-1 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human Cenexin-1 pS796 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Positive Control
ODF2 Lysate (NBL1-13908)

Reactivity Notes

Human, Chimpanzee, Macaque.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ODF2 [p Ser796] Antibody

  • Cenexin
  • FLJ44866
  • MGC111096
  • MGC9034
  • ODF2/1
  • ODF2/2
  • ODF8484-kD
  • outer dense fiber of sperm tails 2
  • outer dense fiber protein 2
  • sperm tail structural protein

Background

Cenexin-1, also known as ODF2 and outer dense fiber of sperm tails 2, are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene; however, not all isoforms and variants have been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ODF2
Entrez
Uniprot