| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85417-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35580-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58793-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22037-50ul | |||
![Western Blot: ODF2 Antibody (1A1) [H00004957-M01] - Detection against Immunogen (36.63 KDa) .](http://images.novusbio.com/fullsize/ODF2-Antibody-1A1-Western-Blot-H00004957-M01-img0006.jpg "Western Blot: ODF2 Antibody (1A1) [H00004957-M01] - Detection against Immunogen (36.63 KDa) .")
![Immunocytochemistry/Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01] - The localization and function of DYF-19 are highly conserved In IMCD3 cells, FBF1 localizes above rootlet and subdistal appendage protein ODF2 and completely colocalizes with distal appendage protein CEP164. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3750) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ODF2-Antibody-1A1-Immunocytochemistry-Immunofluorescence-H00004957-M01-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01] - Co-staining of ODF2 (Cy3), DAPI and r-tubulin (Alexa Fluor 488) in RPE1 cell line. ODF2 stains the subdistal appendage of mother centriole. Cells were fixed with cold methanol, 10min. Blocked with 3% BSA for 30min at room temperature. ODF2 antibody was used at 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/ODF2-Antibody-1A1-Immunocytochemistry-Immunofluorescence-H00004957-M01-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01] - Analysis of monoclonal antibody to ODF2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ODF2-Antibody-1A1-Immunocytochemistry-Immunofluorescence-H00004957-M01-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ODF2 Antibody (1A1) [H00004957-M01] - The localization & function of DYF-19 are highly conservedEither endogenous (a) or overexpressed (b) FBF1, the mammalian homolog of worm DYF-19, localizes specifically on one centriole with a ring-like pattern in IMCD3 cells. c, FBF1 localizes at the ciliary base, above the basal body. d, In IMCD3 cells, FBF1 localizes above rootlet & subdistal appendage protein ODF2 & completely colocalizes with distal appendage protein CEP164. e, Immuno-EM demonstrates that FBF1 localizes specifically to distal appendages of mother centrioles. f–h, Knock-down of FBF1 leads to severely truncated cilia in most RNAi-treated hTERT-RPE cells. Data are represented as mean of 3 independent experiments (n=200) & error bars indicate s.d. Significant differences were identified by the Student’s t-test. *P<0.001. i & j, The IFT-B component IFT88, but not the IFT-A component IFT140, enters the truncated cilia of FBF1-knockdown hTERT-RPE cells. Arrows indicate the tips of truncated cilia. k, Endogenous IFT54 immunoprecipitates with FBF1 in hTERT-RPE cells. l, HEK293 cells were transiently transfected with FLAG-HA-tagged FBF1, & 48 hours later, cells were subjected to immunoprecipitation using normal mouse IgG (mIgG) or anti-IFT54 antibody. 50 μg protein were loaded into each lane. Bars: c, 1μm; e, 200 nm; others, 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24231678), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004957-m01_mouse-monoclonal-odf2-antibody-1a1-310202415482244.jpg)
![Immunohistochemistry-Paraffin: ODF2 Antibody [NBP1-85417] - Staining in human testis and prostate tissues using anti-ODF2 antibody. Corresponding ODF2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ODF2-Antibody-Immunohistochemistry-Paraffin-NBP1-85417-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ODF2 Antibody [NBP1-85417] - Staining in human testis and prostate tissues using anti-ODF2 antibody. Corresponding ODF2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ODF2 Antibody [NBP1-85417] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/fullsize/ODF2-Antibody-Immunohistochemistry-Paraffin-NBP1-85417-img0009.jpg)
![Immunohistochemistry-Paraffin: ODF2 Antibody [NBP1-85417] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/ODF2-Antibody-Immunohistochemistry-Paraffin-NBP1-85417-img0010.jpg)
![Western Blot: ODF2 [p Ser796] Antibody [NB110-85525] - Western Blot of ODF2 [p Ser796] antibody. Lane 1: MW.Lane 2: Human Semen Lysate.Lane 3: MCF7 WCL. Lane 4: Molt 4 WCL. Lane 5: HeLa WCL. Load: 10ug per lane. Blocking:. Primary Antibody: Anti-ODF2 pS796 at 1ug/mL overnight at 2-8C.Secondary Antibody: Goat anti-Rabbit HRP 1:40,000 diluted in for 30 minutes at RT.Expect: 93kDa.](http://images.novusbio.com/fullsize/ODF2-[p-Ser796]-Antibody-Western-Blot-NB110-85525-img0005.jpg "Western Blot: ODF2 [p Ser796] Antibody [NB110-85525] - Western Blot of ODF2 [p Ser796] antibody. Lane 1: MW.Lane 2: Human Semen Lysate.Lane 3: MCF7 WCL. Lane 4: Molt 4 WCL. Lane 5: HeLa WCL. Load: 10ug per lane. Blocking:. Primary Antibody: Anti-ODF2 pS796 at 1ug/mL overnight at 2-8C.Secondary Antibody: Goat anti-Rabbit HRP 1:40,000 diluted in for 30 minutes at RT.Expect: 93kDa.")
![ELISA: ODF2 [p Ser796] Antibody [NB110-85525] - ELISA of ODF2 [p Ser796] antibody. Antigen: BSA conjugates of ODF2 pS796 (purple) and S796 (blue).Coating amount: 0.1 ug per well. Primary antibody: ODF2 at 5 ug/mL. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody at 1:8,500. Substrate: TMB.](http://images.novusbio.com/fullsize/ODF2-[p-Ser796]-Antibody-ELISA-NB110-85525-img0006.jpg)
![Western Blot: ODF2 Antibody [NBP3-35580] - Western blot analysis of various lysates using ODF2 Rabbit pAb at dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-35580_rabbit-odf2-pab-912202415121326.jpg "Western Blot: ODF2 Antibody [NBP3-35580] - Western blot analysis of various lysates using ODF2 Rabbit pAb at dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.")
![Immunocytochemistry/ Immunofluorescence: ODF2 Antibody [NBP3-35580] - Immunofluorescence analysis of HeLa cells using ODF2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35580_rabbit-odf2-pab-91220241418142.jpg)
![Immunocytochemistry/Immunofluorescence: ODF2 Antibody [NBP2-58793] - Staining of human cell line U-2 OS shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ODF2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58793-img0001.jpg "Immunocytochemistry/Immunofluorescence: ODF2 Antibody [NBP2-58793] - Staining of human cell line U-2 OS shows localization to centrosome. Antibody staining is shown in green.")
![Western Blot: ODF2 Antibody [NBP1-54850] - HepG2 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/ODF2-Antibody-Western-Blot-NBP1-54850-img0002.jpg "Western Blot: ODF2 Antibody [NBP1-54850] - HepG2 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: ODF2 Antibody (3Z1H8) [NBP3-16233] - Western blot analysis of extracts of various cell lines, using ODF2 antibody (NBP3-16233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ODF2-Antibody-3Z1H8-Western-Blot-NBP3-16233-img0001.jpg "Western Blot: ODF2 Antibody (3Z1H8) [NBP3-16233] - Western blot analysis of extracts of various cell lines, using ODF2 antibody (NBP3-16233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: ODF2 Antibody (SR2297) [NBP3-22037] - Western blot analysis using NBP3-22037 in Hela cell lysate.](http://images.novusbio.com/fullsize/nbp3-22037_rabbit-odf2-mab-sr2297-57202314565411.jpg "Western Blot: ODF2 Antibody (SR2297) [NBP3-22037] - Western blot analysis using NBP3-22037 in Hela cell lysate.")
![Western Blot: ODF2 Antibody (JE35-01) [NBP3-32676] - Western blot analysis of ODF2 on different lysates with Rabbit anti-ODF2 antibody (NBP3-32676) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: K-562 cell lysate Lane 3: MCF7 cell lysate Lane 4: HeLa cell lysate Lane 5: U-2 OS cell lysate Lane 6: Jurkat cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32676) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32676_rabbit-odf2-mab-je35-01-278202416285286.jpg "Western Blot: ODF2 Antibody (JE35-01) [NBP3-32676] - Western blot analysis of ODF2 on different lysates with Rabbit anti-ODF2 antibody (NBP3-32676) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: K-562 cell lysate Lane 3: MCF7 cell lysate Lane 4: HeLa cell lysate Lane 5: U-2 OS cell lysate Lane 6: Jurkat cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32676) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")