ODF2 Antibody (JE35-01)

Western Blot: ODF2 Antibody (JE35-01) [NBP3-32676] - Western blot analysis of ODF2 on different lysates with Rabbit anti-ODF2 antibody (NBP3-32676) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: K-562 cell lysate Lane 3: MCF7 cell lysate Lane 4: HeLa cell lysate Lane 5: U-2 OS cell lysate Lane 6: Jurkat cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32676) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu
• Applications: WB
• Clone: JE35-01
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ODF2 Antibody (JE35-01) Summary

• Description: Novus Biologicals Rabbit ODF2 Antibody (JE35-01) (NBP3-32676) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human ODF2 aa 780-829 / 829. (Uniprot: Q5BJF6)
• Localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cell projection, cilium, microtubule organizing center, centriole, spindle pole, flagellum.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ODF2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ODF2 Antibody (JE35-01)

• Cenexin
• FLJ44866
• MGC111096
• MGC9034
• ODF2/1
• ODF2/2
• ODF8484-kD
• outer dense fiber of sperm tails 2
• outer dense fiber protein 2
• sperm tail structural protein

Background

Cenexin-1, also known as ODF2 and outer dense fiber of sperm tails 2, are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene; however, not all isoforms and variants have been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ODF2