ODF2 Antibody (3Z1H8)

Western Blot: ODF2 Antibody (3Z1H8) [NBP3-16233] - Western blot analysis of extracts of various cell lines, using ODF2 antibody (NBP3-16233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

• Reactivity: Hu
• Applications: WB
• Clone: 3Z1H8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ODF2 Antibody (3Z1H8) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 730-829 of human ODF2 (Q5BJF6). KEHALSKERAAQNKILDLETQLSRTKTELSQLRRSRDDADRRYQSRLQDLKDRLEQSESTNRSMQNYVQFLKSSYANVFGDGPYSTFLTSSPIRSRSPPA
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ODF2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ODF2 Antibody (3Z1H8)

• Cenexin
• FLJ44866
• MGC111096
• MGC9034
• ODF2/1
• ODF2/2
• ODF8484-kD
• outer dense fiber of sperm tails 2
• outer dense fiber protein 2
• sperm tail structural protein

Background

Cenexin-1, also known as ODF2 and outer dense fiber of sperm tails 2, are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene; however, not all isoforms and variants have been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ODF2