OCT2 Antibody (Oct2/2137)

Images

 
Western Blot: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Analysis (A) Recombinant Protein (B) Daudi cell lysate Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
Immunohistochemistry-Paraffin: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Formalin-fixed, paraffin-embedded human Tonsil stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
Western Blot: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Analysis Ramos cell lysate using Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
Immunohistochemistry-Paraffin: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Formalin-fixed, paraffin-embedded human Lymph Node stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
Protein Array: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Analysis of Protein Array containing more than 19,000 full-length human proteins using OCT2 Antibody (Oct2/2137). Z- and S- Score: The Z-score represents the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, PA
Clone
Oct2/2137
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

OCT2 Antibody (Oct2/2137) Summary

Immunogen
Recombinant fragment of human OCT2 protein (around aa 112-297) (exact sequence is proprietary)
Localization
Nuclear
Specificity
OCT2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. OCT2 expression can be used as a marker of B-cell lineage and differentiation. Germinal center B-cells, mantle B-cells, monocytoid B-cells, and plasma cells show high level expression of OCT2. Additionally, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, Hodgkin lymphoma display increased expression of OCT2. Several studies of OCT2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines have high levels of expression. In spite of scanty evidence for OCT2 expression in T cells, it is believed that this factor participates in transcriptional regulation during T-cell activation.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POU2F2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot : 2 ug/ml
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin : 1-2 ug/ml
  • Protein Array
Application Notes
Positive Control: Raji or U-937 cells. Spleen or Tonsil. ELISA: For coating, order BSA Free version of this antibody. Immunohistochemistry-Paraffin: 1-2 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for OCT2 Antibody (Oct2/2137)

  • homeobox protein
  • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2
  • OCT2Oct-2
  • Octamer-binding protein 2
  • Octamer-binding transcription factor 2
  • OTF-2
  • OTF2POU domain class 2, transcription factor 2
  • POU class 2 homeobox 2
  • POU domain, class 2, transcription factor 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-75735AF350
Alexa Fluor 405 NBP2-75735AF405
Alexa Fluor 488 NBP2-75735AF488
Alexa Fluor 532 NBP2-75735AF532
Alexa Fluor 594 NBP2-75735AF594
Alexa Fluor 647 NBP2-75735AF647
Alexa Fluor 700 NBP2-75735AF700
Alexa Fluor 750 NBP2-75735AF750
Biotin NBP2-75735B
DyLight 350 NBP2-75735UV
DyLight 405 NBP2-75735V
DyLight 488 NBP2-75735G
DyLight 550 NBP2-75735R
DyLight 594 NBP2-75735DL594
DyLight 650 NBP2-75735C
DyLight 680 NBP2-75735FR
DyLight 755 NBP2-75735IR
FITC NBP2-75735F
HRP NBP2-75735H
Janelia Fluor 549 NBP2-75735JF549
Janelia Fluor 646 NBP2-75735JF646

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Bioinformatics

Gene Symbol POU2F2
Uniprot