OCIL/CLEC2d Antibody [Unconjugated]

Raji human Burkitt's lymphoma cell line was stained with Goat Anti-Human OCIL/CLEC2d Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3480, filled histo­gram) or control antibody (Catalog # AB-108-C, open histo­gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

LLT1 is highly expressed in immune-privileged sites.Lectin-like transcript 1 (LLT1) and isotype control stainings of testes (A), brain (B) and placenta (C) using the anti-LLT1 2H7 mAb (1/500). Representative image of three independent experiments. 20× magnification. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28413611), licensed under a CC-BY license. Not internally tested by R&D Systems.

LLT1 is highly expressed in immune-privileged sites.Lectin-like transcript 1 (LLT1) and isotype control stainings of testes (A), brain (B) and placenta (C) using the anti-LLT1 2H7 mAb (1/500). Representative image of three independent experiments. 20× magnification. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28413611), licensed under a CC-BY license. Not internally tested by R&D Systems.

LLT1 is expressed on B cells and monocytes.Lectin-like transcript 1 (LLT1) (stained using the 2H7 mAb) and CD161 levels were measured by flow cytometry in monocytes (M) and lymphocytes (L). (A) The gating strategy, (B) representative and cumulative data for the expression of CD161 (blue) and LLT1 (green) compared to the isotype control (IC, red) (n=4). (C) Representative image of LLT1 staining in human tonsil tissue using the 2H7 anti-LLT1 antibody (10×; n=6), together with a representative FACS plot of LLT1 staining of purified tonsillar B cells with the 2H7 antibody. The GC population is highlighted (rectangle; n=8). (D) Immunofluorescent co-staining of LLT1 (red) and CD68 (green) in lung and liver (scale bar = 100μm). Representative of two independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28413611), licensed under a CC-BY license. Not internally tested by R&D Systems.

LLT1 is highly expressed in immune-privileged sites.Lectin-like transcript 1 (LLT1) and isotype control stainings of testes (A), brain (B) and placenta (C) using the anti-LLT1 2H7 mAb (1/500). Representative image of three independent experiments. 20× magnification. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28413611), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

OCIL/CLEC2d Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human OCIL/CLEC2d
  Ser57-Val191
  Accession # Q9UHP7
• Specificity: Detects human OCIL/CLEC2d in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse (rm) OCIL is observed and less than 1% cross-reactivity with recombinant human (rh) CLEC2B, and rhCLEC2A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLEC2D
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OCIL/CLEC2d Antibody [Unconjugated]

• CLAXLLT-1
• CLEC2D
• C-type lectin domain family 2 member D
• C-type lectin domain family 2, member D
• C-type lectin related f
• Lectin-like NK cell receptor
• Lectin-like transcript 1
• LLT1
• LLT1Osteoclast inhibitory lectin
• OCIL
• OCILmember D

Background

Human OCIL/CLEC2d, also known as Lectin-Like Transcript-1 (LLT-1), is a type II transmembrane protein belonging to the natural killer (NK) cell receptor group of the C-type lectin superfamily. It is found on hematopoietic cells, osteoblasts, and chondrocytes. By alternative splicing, at least three isoforms exist. Isoform 1 is synthesized as a 191 amino acid (aa) precursor with a C-terminal 132 aa extracellular domain (ECD) that contains the C-type lectin domain. The aa sequence of human OCIL isoform 1 ECD is 49% and 50% identical to the mouse and rat OCIL ECD, respectively. Human OCIL preferentially binds high molecular weight sulfated glycosaminoglycans and is a ligand for the single human NKR-P1A receptor (CD161). Human OCIL blocks osteoclast differentiation. Engagement of OCIL with CD161 on NK cells also inhibits NK cell-mediated cytotoxicity and IFN-gamma secretion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OCIL/CLEC2d Antibody (AF3480)(2)

Publications using AF3480

• A Llibre, L Garner, A Partridge, GJ Freeman, P Klenerman, CB Willberg Expression of lectin-like transcript-1 in human tissues F1000Res, 2016-12-29;5(0):2929. 2016-12-29 [PMID: 28413611] (IHC, Human)
• Alba Llibre, Constantino López-Macías, Teresa Marafioti, Hema Mehta, Amy Partridge, Carina Kanzig et al. LLT1 and CD161 Expression in Human Germinal Centers Promotes B Cell Activation and CXCR4 Downregulation The Journal of Immunology 2016-03-01 [PMID: 26829983]

Bioinformatics

• Gene Symbol: CLEC2D
• Uniprot:
  • Q9UHP7()