OCIL/CLEC2d Antibody (402659) [Unconjugated]

Detection of OCIL/CLEC2d in Ramos cells by Flow Cytometry Ramos cells were stained with Mouse Anti-Human OCIL/CLEC2d Monoclonal Antibody (Catalog # MAB3480, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 402659
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

OCIL/CLEC2d Antibody (402659) [Unconjugated] Summary

• Immunogen: E. coli-derived rhOCIL and human OCIL/BaF3 transfectants
  Ser57-Val191
  Accession # NP_037401
• Specificity: Detects rhOCIL in direct ELISAs and Western blots. Stains human OCIL transfectants but not irrelevant transfectants. In Western blots, shows no cross-reactivity with rhCLEC4D or rmOCILrp1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in with Trehalose.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OCIL/CLEC2d Antibody (402659) [Unconjugated]

• CLAXLLT-1
• CLEC2D
• C-type lectin domain family 2 member D
• C-type lectin domain family 2, member D
• C-type lectin related f
• Lectin-like NK cell receptor
• Lectin-like transcript 1
• LLT1
• LLT1Osteoclast inhibitory lectin
• OCIL
• OCILmember D

Background

Human OCIL (osteoclast inhibitory lectin; also LLT1 and CLEC-2D) is a member of the NK cell receptor group of the C-type lectin superfamily. It is a type II transmembrane protein 191 amino acids (aa) in length that contains a C-terminal 132 aa extracellular domain (ECD). Within the ECD lies a C-type lectin domain (aa 75-186). There are at least two alternate splice forms of OCIL. Both exhibit splicing in the ECD. One shows a 40 aa substitution for the C-terminal 37 amino acids. A second shows a 3 aa substitution for the C-terminal 72 amino acids. The ECD of human OCIL is 49% and 50% aa identical to the ECD in mouse and rat OCIL ECD, respectively. The molecule is found on hematopoietic cells, osteoblasts and chondrocytes. It binds sulfated GAGs and NKR-P1B and D receptors, and blocks osteoclast formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OCIL/CLEC2d Antibody (MAB3480)(2)

Publications using MAB3480

• Germain C, Meier A, Jensen T, Knapnougel P, Poupon G, Lazzari A, Neisig A, Hakansson K, Dong T, Wagtmann N, Galsgaard ED, Spee P, Braud VM Induction of lectin-like transcript 1 (LLT1) protein cell surface expression by pathogens and interferon-gamma contributes to modulate immune responses. J. Biol. Chem., 2011-09-19;286(44):37964-75. 2011-09-19 [PMID: 21930700] (Neutralization, Human)
• Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells. Int. J. Cancer, 2009-04-01;124(7):1594-604. 2009-04-01 [PMID: 19089914] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • NP_037401()