OCIL/CLEC2d Antibody (402659) [Phycoerythrin]

Ramos cells were stained with Mouse Anti-Human OCIL/CLEC2d PE‑conjugated Monoclonal Antibody (Catalog # FAB3480P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 402659
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

OCIL/CLEC2d Antibody (402659) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human OCIL/CLEC2d
  Ser57-Val191
  Accession # Q9UHP7
• Specificity: Detects human OCIL/CLEC2d.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC2D
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OCIL/CLEC2d Antibody (402659) [Phycoerythrin]

• CLAXLLT-1
• CLEC2D
• C-type lectin domain family 2 member D
• C-type lectin domain family 2, member D
• C-type lectin related f
• Lectin-like NK cell receptor
• Lectin-like transcript 1
• LLT1
• LLT1Osteoclast inhibitory lectin
• OCIL
• OCILmember D

Background

Human OCIL (Osteoclast Inhibitory Lectin), also known as LLT1 and CLEC-2D, is a member of the NK cell receptor group of the C-type lectin superfamily. It is a type II transmembrane protein 191 amino acids (aa) in length that contains a C-terminal 132 aa extracellular domain (ECD). Within the ECD lies a C-type lectin domain (aa 75-186). There are at least two alternate splice forms of OCIL. Both exhibit splicing in the ECD. One shows a 40 aa substitution for the C-terminal 37 amino acids. A second shows a 3 aa substitution for the C-terminal 72 amino acids. The ECD of human OCIL is 49% and 50% aa identical to the ECD in mouse and rat OCIL ECD, respectively. The molecule is found on hematopoietic cells, osteoblasts and chondrocytes. It binds sulfated GAGs and NKR-P1B and D receptors, and blocks osteoclast formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OCIL/CLEC2d Antibody (FAB3480P)(5)

Publications using FAB3480P

• Liyan Lao, Wenfeng Zeng, Penghan Huang, Huiping Chen, Zishuo Jia, Pei Wang et al. CD8+ T cell–Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance Cancer Immunology Research 2023-03-01 [PMID: 36603133]
• Marrufo A, Mathew S, Chaudhary P, Malaer J, Vishwanatha J, Mathew P Blocking LLT1 (CLEC2D, OCIL)-NKRP1A (CD161) interaction enhances natural killer cell-mediated lysis of triple-negative breast cancer cells. Am J Cancer Res, 2018-06-01;8(6):1050-1063. 2018-06-01 [PMID: 30034942] (Flow Cytometry, ICC, Human)
• Clark GJ et al. New insights into the phenotype of human dendritic cell populations. Clin Transl Immunology 1/1/2016 [PMID: 26900474]
• Chalan P, Bijzet J, Huitema M, Kroesen B, Brouwer E, Boots A Expression of Lectin-Like Transcript 1, the Ligand for CD161, in Rheumatoid Arthritis. PLoS ONE, 2015-07-06;10(7):e0132436. 7/6/2015 [PMID: 26147876]
• Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma. PLoS Pathog., 2012-01-12;8(1):e1002486. 2012-01-12 [PMID: 22253598] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CLEC2D
• Uniprot:
  • Q9UHP7()