Occludin Antibody (1G7) - Azide and BSA Free

Western Blot: Occludin Antibody (1G7) [H00004950-M01] - OCLN monoclonal antibody (M01), clone 1G7 Analysis of OCLN expression in HL-60.

Immunocytochemistry/Immunofluorescence: Occludin Antibody (1G7) [H00004950-M01] - Analysis of monoclonal antibody to OCLN on HeLa cell. Antibody concentration 10 ug/ml.

Immunoprecipitation: Occludin Antibody (1G7) [H00004950-M01] - Analysis of OCLN transfected lysate using anti-OCLN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with OCLN MaxPab rabbit polyclonal antibody.

ELISA: Occludin Antibody (1G7) [H00004950-M01] - Detection limit for recombinant GST tagged OCLN is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 1G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Occludin Antibody (1G7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: OCLN (NP_002529, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT
• Marker: Tight Junctions Marker
• Specificity: OCLN - occludin
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: OCLN
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Occludin Antibody (1G7) - Azide and BSA Free

• BLCPMG
• Occludin
• OCLN

Background

This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Occludin Antibody (H00004950-M01)(5)

Publications using H00004950-M01

• Islam MJ, Amin MB, Uddin MKM et al. Mouse homologues of hepatitis C virus human entry factors inhibit the entry of HCV pseudoparticles (HCVpp) into human hepatoma cells. Bioresearch Communications 2016-01-01 (WB, Human)
• Beata B, Lilla E, Edit U et al. Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures. Sci Rep. 2020-07-30 [PMID: 32733073]
• Struver K, Friess W, Hedtrich S. et al. Development of a Perfusion Platform for Dynamic Cultivation of in vitro Skin Models. Skin Pharmacol Physiol 2017-06-27 [PMID: 28651246]
• Bambang IF, Lee YK, Richardson DR et al. Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells. Oncogene 2012-04-30 [PMID: 22543584] (Human)
• Jevtic M Fine-Tuning of Organotypic Skin Equivalents for Preclinical Research and Their Utilization to Study Epidermal-Dermal Crosstalk Thesis 2021-01-01

Bioinformatics

• Gene Symbol: OCLN
• Entrez:
  • 4950(Human)
• OMIM:
  • 602876(Human)
• Uniprot:
  • NP_002529(Human)