OCAM/NCAM2 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

OCAM/NCAM2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse OCAM
  Leu20-Gly700
  Accession # O35136
• Specificity: Detects mouse OCAM in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) NCAM-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NCAM2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OCAM/NCAM2 Antibody [Unconjugated]

• NCAM2
• N-CAM-2
• NCAM-2
• NCAM21MGC51008
• neural cell adhesion molecule 2 precursor10
• neural cell adhesion molecule 2
• OCAM
• Rb-8
• RNCAM

Background

OCAM is a homophilic adhesion molecule belonging to the immunoglobulin superfamily and is expressed by a subset of both olfactory and vomeronasal axons in a zone-specific manner. OCAM is also known as neural cell adhesion molecule 2 (NCAM-2) and Rb-8 neural cell adhesion molecule (RNCAM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OCAM/NCAM2 Antibody (AF778)(15)

Publications using AF778

• Ueha R, Ito T, Furukawa R et al. Oral SARS-CoV-2 Inoculation Causes Nasal Viral Infection Leading to Olfactory Bulb Infection: An Experimental Study Frontiers in Cellular and Infection Microbiology 2022-06-13 [PMID: 35770069]
• M Aoki, I Gamayun, A Wyatt, R Grünewald, M Simon-Thom, SE Philipp, O Hummel, S Wagenpfeil, K Kattler, G Gasparoni, J Walter, S Qiao, DR Grattan, U Boehm Prolactin-sensitive olfactory sensory neurons regulate male preference in female mice by modulating responses to chemosensory cues Science Advances, 2021-10-08;7(41):eabg4074. 2021-10-08 [PMID: 34623921] (IHC, Mouse)
• A Parcerisas, A Ortega-Gas, M Hernaiz-Ll, MA Odena, F Ulloa, E de Oliveir, M Bosch, L Pujadas, E Soriano New Partners Identified by Mass Spectrometry Assay Reveal Functions of NCAM2 in Neural Cytoskeleton Organization International Journal of Molecular Sciences, 2021-07-09;22(14):. 2021-07-09 [PMID: 34299022] (Immunoprecipitation, Mass Spectrometry, Western Blot, Mouse)
• W Kim, H Watanabe, S Lomoio, G Tesco Spatiotemporal processing of neural cell adhesion molecules 1 and 2 by BACE1 in vivo The Journal of Biological Chemistry, 2021-02-03;0(0):100372. 2021-02-03 [PMID: 33548223] (IHC, Mouse)
• Lemons K, Fu Z, Ogura T, Lin W TRPM5-expressing Microvillous Cells Regulate Region-specific Cell Proliferation and Apoptosis During Chemical Exposure Neuroscience 3/26/2020 [PMID: 32224228]
• Uyen P. Nguyen, Fumiaki Imamura Regional differences in mitral cell development in mouse olfactory bulb Journal of Comparative Neurology 2019-03-22 [PMID: 30864157]
• JH Coleman, B Lin, JD Louie, J Peterson, RP Lane, JE Schwob SPATIAL DETERMINATION OF NEURONAL DIVERSIFICATION IN THE OLFACTORY EPITHELIUM J. Neurosci., 2018-12-10;0(0):. 2018-12-10 [PMID: 30530861] (IHC-Fr, Mouse)
• Martina Pyrski, Mahbuba Tusty, Eugenia Eckstein, Livio Oboti, Diego J. Rodriguez-Gil, Charles A. Greer et al. P/Q Type Calcium Channel Cav2.1 Defines a Unique Subset of Glomeruli in the Mouse Olfactory Bulb Frontiers in Cellular Neuroscience 2018-09-04 [PMID: 30233329]
• LI Korsak, KA Shepard, MR Akins Cell type-dependent axonal localization of translational regulators and mRNA in mouse peripheral olfactory neurons J. Comp. Neurol, 2017-03-26;0(0):. 2017-03-26 [PMID: 28266018] (IHC, Mouse)
• Eerdunfu, N Ihara, B Ligao, Y Ikegaya, H Takeuchi Differential timing of neurogenesis underlies dorsal-ventral topographic projection of olfactory sensory neurons Neural Dev, 2017-02-13;12(1):2. 2017-02-13 [PMID: 28193234] (IHC, Mouse)
• Deleyrolle L, Sabourin J, Rothhut B, Fujita H, Guichet P, Teigell M, Ripoll C, Chauvet N, Perrin F, Mamaeva D, Noda T, Mori K, Yoshihara Y, Hugnot J OCAM Regulates Embryonic Spinal Cord Stem Cell Proliferation by Modulating ErbB2 Receptor. PLoS ONE, 2015-04-13;10(4):e0122337. 2015-04-13 [PMID: 25875008] (Bioassay, Flow Cytometry, IHC, Western Blot, Mouse)
• Carolyn E. Peluso, Woochan Jang, Ursula C. Dräger, James E. Schwob Differential expression of components of the retinoic acid signaling pathway in the adult mouse olfactory epithelium The Journal of Comparative Neurology 2012-11-01 [PMID: 22522724]
• Miyamichi K, Amat F, Moussavi F Cortical representations of olfactory input by trans-synaptic tracing. Nature, 2010-12-22;472(7342):191-6. 2010-12-22 [PMID: 21179085] (IHC, Mouse)
• Murdoch B, Roskams AJ A novel embryonic nestin-expressing radial glia-like progenitor gives rise to zonally restricted olfactory and vomeronasal neurons. J. Neurosci., 2008-04-16;28(16):4271-82. 2008-04-16 [PMID: 18417707] (IHC-Fr, Mouse)
• Zou DJ, Chesler AT, Le Pichon CE, Kuznetsov A, Pei X, Hwang EL, Firestein S Absence of adenylyl cyclase 3 perturbs peripheral olfactory projections in mice. J. Neurosci., 2007-06-20;27(25):6675-83. 2007-06-20 [PMID: 17581954] (IHC, Mouse)

Bioinformatics

• Gene Symbol: NCAM2
• Uniprot:
  • O35136()