Recombinant Human OBFC1 His Protein

SDS-Page: OBFC1 Protein [NBP2-23308]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human OBFC1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-368 of Human OBFC1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQPGSSR CEEETPSLLW GLDPVFLAFA KLYIRDILDM KESRQVPGVF LYNGHPIKQV DVLGTVIGVR ERDAFYSYGV DDSTGVINCI CWKKLNTESV SAAPSAAREL SLTSQLKKLQ ETIEQKTKIE IGDTIRVRGS IRTYREEREI HATAYYKVDD PVWNIQIARM LELPTIYRKV YDQPFHSSAL EKEEALSNPG ALDLPSLTSL LSEKAKEFLM ENRVQSFYQQ ELEMVESLLS LANQPVIHSA CSDQVNFKKD TTSKAIHSIF KNAIQLLQEK GLVFQKDDGF DNLYYVTRED KDLHRKIHRI IQQDCQKPNH MEKGCHFLHI LACARLSIRP GLSEAVLQQV LELLEDQSDI VSTMEHYYTA F

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: STN1
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 44.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human OBFC1 His Protein

• AAF44
• AAF-44
• alpha accessory factor 44
• bA541N10.2
• CST complex subunit STN1
• oligonucleotide/oligosaccharide-binding fold containing 1
• Oligonucleotide/oligosaccharide-binding fold-containing protein 1
• replication protein A 32 kDa subunit
• RP11-541N10.2
• RPA-32
• STN1FLJ22559
• Suppressor of cdc thirteen homolog

Background

CST complex subunit STN1, also known as OBFC1, is a component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. Recombinant human OBFC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: STN1