OBFC1 Antibody (3G12-1B7)

Western Blot: OBFC1 Antibody (3G12-1B7) [H00079991-M01] - OBFC1 monoclonal antibody (M01), clone 3G12-1B7 Analysis of OBFC1 expression in Jurkat.

Product Discontinued

OBFC1 Antibody (3G12-1B7) Summary

• Immunogen: OBFC1 (AAH17400, 1 a.a. ~ 368 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQPGSSRCEEETPSLLWGLDPVFLAFAKLYIRDILDMKESRQVPGVFLYNGHPIKQVDVLGTVIGVRERDAFYSYGVDDSTGVINCICWKKLNTESVSAAPSAARELSLTSQLKKLQETIEQKTKIEIGDTIRVRGSIRTYREEREIHATAYYKVDDPVWNIQIARMLELPTIYRKVYDQPFHSSALEKEEALSNPGALDLPSLTSLLSEKAKEFLMENRVQSFYQQELEMVESLLSLANQPVIHSACSDQVNFKKDTTSKAIHSIFKNAIQLLQEKGLVFQKDDGFDNLYYVTREDKDLHRKIHRIIQQDCQKPNHMEKGCHFLHILACARLSIRPGLSEAVLQQVLELLEDQSDIVSTMEHYYTAF
• Specificity: OBFC1 - oligonucleotide/oligosaccharide-binding fold containing 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STN1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for OBFC1 Antibody (3G12-1B7)

• AAF44
• AAF-44
• alpha accessory factor 44
• bA541N10.2
• CST complex subunit STN1
• oligonucleotide/oligosaccharide-binding fold containing 1
• Oligonucleotide/oligosaccharide-binding fold-containing protein 1
• replication protein A 32 kDa subunit
• RP11-541N10.2
• RPA-32
• STN1FLJ22559
• Suppressor of cdc thirteen homolog

Background

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNApolymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 (PubMed19119139)). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM613130) (Miyake et al., 2009 (PubMed 19854130)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OBFC1 Antibody (H00079991-M01)(1)

Publication using H00079991-M01

• Wan M, Qin J, Songyang Z et al. OB fold-containing protein 1 (OBFC1), a human homolog of yeast Stn1, associates with TPP1 and is implicated in telomere length regulation. J Biol Chem 2009-09-01 [PMID: 19648609]

Bioinformatics

• Gene Symbol: STN1
• Entrez:
  • 79991(Human)
• Uniprot:
  • AAH17400(Human)