NUP98 Antibody (2H4) - BSA Free

Western Blot: NUP98 Antibody (2H4) [NBP3-15200] - Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate. All lanes: NUP98 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 198, 188, 98, 97, 196, 187 KDa. Observed band size: 100 KDa.

Immunocytochemistry/Immunofluorescence: NUP98 Antibody (2H4) [NBP3-15200] - Immunofluorescence staining of Hela Cells with at NUP98 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry: NUP98 Antibody (2H4) [NBP3-15200] - IHC image of NUP98 antibody diluted at 1:100 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2H4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NUP98 Antibody (2H4) - BSA Free Summary

• Description: Novus Biologicals Rabbit NUP98 Antibody (2H4) - BSA Free (NBP3-15200) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human NUP98. UniProt ID (P52948).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NUP98
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NUP98 Antibody (2H4) - BSA Free

• ADAR2
• ADIR2
• GLFG-repeat containing nucleoporin
• nuclear pore complex protein Nup98-Nup96
• nucleoporin 98kD
• nucleoporin 98kDa
• NUP196
• NUP96
• Nup98-Nup96

Background

Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kD nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kD precursor protein. This cleavage results in the 98 kD nucleoporin as well as a 96 kD nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kD nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromsome translocatons in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUP98