NUP50 Antibody

Western Blot: NUP50 Antibody [NB100-1212] - Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: NUP50 Antibody [NB100-1212] - 3.8ug/ml staining of Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NUP50 Antibody Summary

• Immunogen: Peptide with sequence C-ELHKILLEKKDA corresponding to C-Terminus according to NP_009103.2, NP_705931.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1).
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NUP50
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.2 - 1 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in Jurkat lysates (calculated MW 50.1 kDa band according to NP_009103.2). IHC-P: Human uterus shows textured nuclear staining of endometrial cells.
• Control:

  Jurkat Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NUP50 Antibody

• 50 kDa nucleoporin
• MGC39961
• NPAP60
• NPAP60LNucleoporin Nup50
• nuclear pore complex protein Nup50
• Nuclear pore-associated protein 60 kDa-like
• nuclear pore-associated protein 60L
• nucleoporin 50kD
• nucleoporin 50kDa

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NUP50 Antibody (NB100-1212)(5)

Publications using NB100-1212

• Gonzalez JM, Navarro-Puche A, Casar B, Crespo P, Andres V. Fast regulation of AP-1 activity through interaction of lamin A/C, ERK1/2, and c-Fos at the nuclear envelope. J Cell Biol;183(4):653-66. 2008-11-17 [PMID: 19015316]
• Shitashige M, Satow R, Honda K et al. Regulation of Wnt signaling by the nuclear pore complex. Gastroenterology. 2008-06-01 [PMID: 18439914] (WB)
  
  Details:
  WB (HCT-116 and DLD1) colon carcinoma cells (Fig 1A, Supplementary Table S1). The IMG-3549 antibody was using in IP/WB where a TCF-4 antibody was used for IP and IMG-3549 was used for WB. The results showed that Npap60/Nup50 co-immunprecipitated with TCF-
• Doufexis M, Storr HL, King PJ, Clark AJ. Interaction of the melanocortin 2 receptor with nucleoporin 50: evidence for a novel pathway between a G-protein-coupled receptor and the nucleus. FASEB J;21(14):4095-100. 2007-12-01 [PMID: 17625072]
• Ivorra C, Kubicek M, Gonzalez JM, Sanz-Gonzalez SM, Alvarez-Barrientos A, O'Connor JE, Burke B, Andres V. A mechanism of AP-1 suppression through interaction of c-Fos with lamin A/C. Genes Dev;20(3):307-20. 2006-02-01 [PMID: 16452503]
• Lindsay ME, Plafker K, Smith AE et al. Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import. Cell 2002-08-09 [PMID: 12176322]

Bioinformatics

• Gene Symbol: NUP50
• Entrez:
  • 10762(Human)
  • 18141(Mouse)
• Uniprot:
  • NP_009103(Human)