NuMA Antibody

Images

 
Western Blot: NuMA Antibody [H00004926-D01P] - Analysis of NUMA1 expression in mouse spleen.
Immunocytochemistry/ Immunofluorescence: NuMA Antibody [H00004926-D01P] - Analysis of antibody to NUMA1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: NuMA Antibody [H00004926-D01P] - Analysis of NUMA1 expression in human spleen.
Western Blot: NuMA Antibody [H00004926-D01P] - Analysis of NUMA1 expression in transfected 293T cell line by NUMA1 polyclonal antibody.Lane 1: NUMA1 transfected lysate(133.20 KDa).Lane 2: Non-transfected lysate.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      H00004926-D01P
    • Availability
      Product Discontinued

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NuMA Antibody Summary

Description
Quality control test: Antibody reactive against mammalian transfected lysate.
Immunogen
NUMA1 (AAH13023.1, 1 a.a. - 1193 a.a.) full-length human protein. MAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGALRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH
Specificity
Reacts with nuclear mitotic apparatus protein 1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUMA1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
This antibody is reactive against tissue and transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NuMA Antibody

  • centrophilin stabilizes mitotic spindle in mitotic cells
  • NMP-22
  • nuclear mitotic apparatus protein 1
  • NuMA protein
  • NuMA
  • NUMA1
  • SP-H Antigen
  • structural nuclear protein

Background

NuMA (nuclear mitotic apparatus) is a long coiled-coil protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning. Therefore, this antibody is useful as a cell cycle marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional NuMA Products

Research Areas for NuMA Antibody (H00004926-D01P)

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Blogs on NuMA.

NuMA: The Key to Asymmetric Cell Division
Nuclear Mitotic Apparatus protein (NuMA) is a cell cycle-related protein that acts as an organizer of the mitotic spindle during mitosis. It may be involved in coordinating the alignment of the mitotic spindle to the cellular polarity axis, which is a...  Read full blog post.

Nucleus and Mitotic Apparatus (NuMA) Protein in Cell Cycle Regulation
NuMA, the major protein of the "Nucleus and Mitotic Apparatus", is a structural protein in vertebrates involved in cell cycle regulation. It localizes to the nucleus during interphase, and accumulates at the spindle poles during mitosis and NuMA has b...  Read full blog post.

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Bioinformatics

Gene Symbol NUMA1
Entrez
Uniprot