Nuf2 Antibody (644) - Azide and BSA Free

Western Blot: Nuf2 Antibody (644) [NBP2-43779] - Analysis of whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDCA1 antibody [644] at a dilution of 1:1000.

Immunoprecipitation: Nuf2 Antibody (644) [NBP2-43779] - Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 ug of NUF2 antibody [644]. Western blot analysis was performed using NUF2 antibody [644]. EasyBlot anti-Mouse IgG was used as a secondary reagent.

• Reactivity: Hu
• Applications: WB, IP
• Clone: 644
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Nuf2 Antibody (644) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Nuf2 Antibody (644) - Azide and BSA Free (NBP2-43779) is a monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein corresponding to human Nuf2
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NUF2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Nuf2 Antibody (644) - Azide and BSA Free

• cancer/testis antigen 106
• CDCA1hsNuf2
• cell division cycle associated 1
• Cell division cycle-associated protein 1
• CT106
• hNuf2
• kinetochore protein Nuf2
• NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
• NUF2RhNuf2R

Background

Nuf2, also known as Kinetochore protein Nuf2, is a 54 kDa 464 amino acid protein, and it is involved in chromosome segregation, specifically as it relates to spindle checkpoint activity with the kinetochore-associated NDC80 complex. This protein is necessary for kinetochore stability and organization of microtubule binding sites on the outer plate, forming interactions with NDC80/HEC-1-CDCA1, SPBC24-SPBC25, Bub1, EXOC1, as well as AURKB/Aurora-B and CENPE in the mitotic prometaphase, DNA replication, and Cell Cycle pathways. Current research is being performed on this proteins involvement in gastric, lung, and breast cancers, in addition to essential hypertension, schizophrenia, and duodenogastric reflux.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUF2