NUDT2 Antibody (4A4-3C3)

Western Blot: NUDT2 Antibody (4A4-3C3) [H00000318-M01] - Analysis of NUDT2 expression in transfected 293T cell line by NUDT2 monoclonal antibody (M01), clone 4A4-3C3.Lane 1: NUDT2 transfected lysate(16.8 KDa).Lane 2: Non-transfected lysate.

Western Blot: NUDT2 Antibody (4A4-3C3) [H00000318-M01] - NUDT2 monoclonal antibody (M01), clone 4A4-3C3 Analysis of NUDT2 expression in Jurkat.

ELISA: NUDT2 Antibody (4A4-3C3) [H00000318-M01] - Detection limit for recombinant GST tagged NUDT2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IHC-P
• Clone: 4A4-3C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NUDT2 Antibody (4A4-3C3) Summary

• Immunogen: NUDT2 (AAH04926, 1 a.a. ~ 147 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALRACGLIIFRRCLIPKVDNNAIEFLLLQASDGIHHWTPPKGHVEPGEDDLETALRETQEEAGIEAGQLTIIEGFKRELNYVARNKPKTVIYWLAEVKDYDVEIRLSHEHQAYRWLGLEEACQLAQFKEMKAALQEGHQFLCSIEA
• Specificity: NUDT2 - nudix (nucleoside diphosphate linked moiety X)-type motif 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NUDT2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NUDT2 Antibody (4A4-3C3)

• Ap4A hydrolase 1
• Ap4A hydrolase
• Ap4Aase
• APAH1Diadenosine 5'-5'''-P1
• bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)
• bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
• diadenosine 5'-5''-P1
• Diadenosine tetraphosphatase
• EC 3.6.1.17
• MGC10404
• Nucleoside diphosphate-linked moiety X motif 2
• nudix (nucleoside diphosphate linked moiety X)-type motif 2
• Nudix motif 2
• P4-tetraphosphate asymmetrical hydrolase
• P4-tetraphosphate pyrophosphohydrolase

Background

This gene encodes a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. The gene product possesses a modification of the MutT sequence motif found in certain nucleotide pyrophosphatases. The enzyme asymmetrically hydrolyzes Ap4A to yield AMP and ATP and is responsible for maintaining the intracellular level of the dinucleotide Ap4A, the function of which has yet to be established. This gene may be a candidate tumor suppressor gene. Alternative splicing has been observed at this locus and three transcript variants, all encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NUDT2 Antibody (H00000318-M01)(1)

Publication using H00000318-M01

• Oka K, Suzuki T, Onodera Y et al. Nudix-type motif 2 (NUDT2) in human breast carcinoma: A potent prognostic factor associated with cell proliferation. Int J Cancer. 2010-06-09 [PMID: 20533549]

Bioinformatics

• Gene Symbol: NUDT2
• Entrez:
  • 318(Human)
• OMIM:
  • 602852(Human)
• Uniprot:
  • AAH04926(Human)