Nudel Antibody

Western Blot: Nudel Antibody [NBP1-28801] - (0.1ug/ml) staining of Human Cer(35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Nudel Antibody Summary

• Description: Novus Biologicals Goat Nudel Antibody (NBP1-28801) is a polyclonal antibody validated for use in WB and ELISA. Anti-Nudel Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CRNFAKDQASRKSY corresponding to internal region according to NP_110435.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001020750.1; NP_110435.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NDEL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 38+39 kDa band bands observed in human brain (cerebellum) lysates (calculated MW of 37.0 kDa band according to NP_001020750.1 and of 38.4 kDa band according to NP_110435.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nudel Antibody

• DKFZp451M0318
• EOPA
• MITAP1mitosin-associated protein MITAP1
• Mitosin-associated protein 1
• nuclear distribution protein nudE-like 1
• nudE nuclear distribution gene E homolog (A. nidulans)-like 1
• nudE nuclear distribution gene E homolog like 1 (A. nidulans)
• nudE nuclear distribution gene E homolog like 1
• NUDELendooligopeptidase A
• NUDE-like protein
• Protein Nudel

Background

This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nudel Antibody (NBP1-28801)(1)

Publication using NBP1-28801

• Yingling J, Youn YH, Darling D et al. Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division. Cell 2008-02-01 [PMID: 18267077]

Bioinformatics

• Gene Symbol: NDEL1
• Entrez:
  • 81565(Human)
  • 83431(Mouse)
  • 170845(Rat)
• Uniprot:
  • NP_001020750.1(Human)