Nucleoporin NUP85 Antibody

Images

 
Western Blot: Nucleoporin NUP85/Pericentrin 1 Antibody [NBP2-31860] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4
Orthogonal Strategies: Immunohistochemistry-Paraffin: Nucleoporin NUP85 Antibody [NBP2-31860] - Nucleoporin NUP85/Pericentrin 1 Antibody [NBP2-31860] - Staining in human testis and skeletal muscle tissues using ...read more
Immunohistochemistry-Paraffin: Nucleoporin NUP85/Pericentrin 1 Antibody [NBP2-31860] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Nucleoporin NUP85/Pericentrin 1 Antibody [NBP2-31860] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Nucleoporin NUP85 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DGEPTVTLIPGVNSKKNQMYFDWGPGEMLVCETSFNKKEKSEMVPSCPFIYIIRKDVDVYSQILRKLFNESHGIFL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUP85
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Nucleoporin NUP85 Recombinant Protein Antigen (NBP2-31860PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Nucleoporin NUP85 Antibody

  • 85 kDa nucleoporin
  • FLJ12549
  • FROUNT
  • nuclear pore complex protein Nup85
  • nucleoporin 85kDa
  • Nucleoporin Nup75
  • Nucleoporin Nup85
  • Nucleoporin
  • Nup75
  • NUP85
  • PCNT
  • PCNT1
  • Pericentrin-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NUP85
Uniprot