Nucleolin Antibody (NCL/902) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity Hu, Bv(-), Mu(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clone
NCL/902
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

Order Details

Nucleolin Antibody (NCL/902) [Alexa Fluor® 700] Summary

Immunogen
Recombinant human Nucleolin protein
Localization
Nucleoli
Marker
Marker of Human Cells
Specificity
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NCL
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Mouse, Rat or Bovine.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Nucleolin Antibody (NCL/902) [Alexa Fluor® 700]

  • C23
  • FLJ45706
  • FLJ59041
  • nucleolin
  • Protein C23

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P, ICC/IF

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47860AF350
Alexa Fluor 405 NBP2-47860AF405
Alexa Fluor 488 NBP2-47860AF488
Alexa Fluor 532 NBP2-47860AF532
Alexa Fluor 594 NBP2-47860AF594
Alexa Fluor 647 NBP2-47860AF647
Alexa Fluor 700 NBP2-47860AF700
Alexa Fluor 750 NBP2-47860AF750
Allophycocyanin NBP2-47860APC
Biotin NBP2-47860B
DyLight 350 NBP2-47860UV
DyLight 405 NBP2-47860V
DyLight 488 NBP2-47860G
DyLight 550 NBP2-47860R
DyLight 594 NBP2-47860DL594
DyLight 650 NBP2-47860C
DyLight 680 NBP2-47860FR
DyLight 755 NBP2-47860IR
FITC NBP2-47860F
HRP NBP2-47860H
Janelia Fluor 549 NBP2-47860JF549
Janelia Fluor 646 NBP2-47860JF646
PE NBP2-47860PE
PerCP NBP2-47860PCP

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Bioinformatics Tool for Nucleolin Antibody (NBP2-47860AF700)

Discover related pathways, diseases and genes to Nucleolin Antibody (NBP2-47860AF700). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Nucleolin.

Nucleolin: A Multifaceted Nucleolar Phosphoprotein
Nucleolin is a ubiquitous, nonhistone nucleolar phosphoprotein of exponentially growing eukaryotic cells and is present in abundance at the dense fibrillar and granular regions of nucleolus. Intact nucleolin is the major species and represents 5% of n...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

Nucleolin: To the Nucleus and Beyond!
Nucleolin is a multifunctional phosphoprotein ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. Nucleolin has a bipartite nuclear localization signal sequence and is conserved across the species. Nucleolin levels are expres...  Read full blog post.

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Bioinformatics

Gene Symbol NCL