Nucleolin Antibody (364-5 + NCL/902) [FITC]

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Product Details

Summary
Reactivity Hu, Bv(-), Mu(-), Rt(-)Species Glossary
Applications Flow, IHC, IHC-P, ICC/IF
Clone
364-5 + NCL/902
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

Nucleolin Antibody (364-5 + NCL/902) [FITC] Summary

Immunogen
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902)
Localization
Nucleoli
Marker
Marker of Human Cells
Specificity
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
NCL
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Nucleolin Antibody (364-5 + NCL/902) [FITC]

  • C23
  • FLJ45706
  • FLJ59041
  • nucleolin
  • Protein C23

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47862AF350
Alexa Fluor 405 NBP2-47862AF405
Alexa Fluor 488 NBP2-47862AF488
Alexa Fluor 532 NBP2-47862AF532
Alexa Fluor 594 NBP2-47862AF594
Alexa Fluor 647 NBP2-47862AF647
Alexa Fluor 700 NBP2-47862AF700
Alexa Fluor 750 NBP2-47862AF750
Allophycocyanin NBP2-47862APC
Allophycocyanin/Cy7 NBP2-47862APCCY7
Biotin NBP2-47862B
DyLight 350 NBP2-47862UV
DyLight 405 NBP2-47862V
DyLight 488 NBP2-47862G
DyLight 550 NBP2-47862R
DyLight 594 NBP2-47862DL594
DyLight 650 NBP2-47862C
DyLight 680 NBP2-47862FR
DyLight 755 NBP2-47862IR
FITC NBP2-47862F
HRP NBP2-47862H
Janelia Fluor 549 NBP2-47862JF549
Janelia Fluor 646 NBP2-47862JF646
PE NBP2-47862PE
PE/Atto594 NBP2-47862PEATT594
PE/Cy5.5 NBP2-47862PECY55
PE/Cy7 NBP2-47862PECY7
PerCP NBP2-47862PCP

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Bioinformatics Tool for Nucleolin Antibody (NBP2-47862F)

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Blogs on Nucleolin.

Nucleolin: A Multifaceted Nucleolar Phosphoprotein
Nucleolin is a ubiquitous, nonhistone nucleolar phosphoprotein of exponentially growing eukaryotic cells and is present in abundance at the dense fibrillar and granular regions of nucleolus. Intact nucleolin is the major species and represents 5% of n...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

Nucleolin: To the Nucleus and Beyond!
Nucleolin is a multifunctional phosphoprotein ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. Nucleolin has a bipartite nuclear localization signal sequence and is conserved across the species. Nucleolin levels are expres...  Read full blog post.

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Bioinformatics

Gene Symbol NCL