Nucleobindin 1 Antibody (1H8-2C11)

Western Blot: Nucleobindin 1 Antibody (1H8-2C11) [H00004924-M01] - NUCB1 monoclonal antibody (M01), clone 1H8-2C11 Analysis of NUCB1 expression in HepG2.

Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody (1H8-2C11) [H00004924-M01] - Analysis of monoclonal antibody to NUCB1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody (1H8-2C11) [H00004924-M01] - Analysis of monoclonal antibody to NUCB1 on formalin-fixed paraffin-embedded human spleen tissue. Antibody concentration 3 ug/ml.

Sandwich ELISA: Nucleobindin 1 Antibody (1H8-2C11) [H00004924-M01] - Detection limit for recombinant GST tagged NUCB1 is approximately 0.1ng/ml as a capture antibody.

Product Discontinued

Nucleobindin 1 Antibody (1H8-2C11) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NUCB1 (AAH02356, 28 a.a. ~ 461 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKSGKLSRELDFVSHHVRTKLDELERQEVSRPRMLLKAKMDAEQDPNVQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRFEEELAAREAELNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQLKFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL
• Specificity: NUCB1 - nucleobindin 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NUCB1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Nucleobindin 1 Antibody (1H8-2C11)

• DKFZp686A15286
• FLJ40471
• NUCCALNUC
• nucleobindin 1
• nucleobindin-1

Background

Nucleobindin 1 is a major calcium binding protein of the Golgi. It may have a role in calcium homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUCB1
• Entrez:
  • 4924(Human)
• OMIM:
  • 601323(Human)
• Uniprot:
  • AAH02356(Human)