Western Blot: NTH1 Antibody [NB100-302] - Analysis of A549 cells using NB100-302. A band is seen at 35 kDa representing NTH1.
Immunocytochemistry/ Immunofluorescence: NTH1 Antibody [NB100-302] - The NTH1 antibody was tested in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more
This NTH1 antibody is useful for Western blot, where a band at ~37 kD. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1% Sodium Azide
Alternate Names for NTH1 Antibody
endonuclease III-like protein 1
nth endonuclease III-like 1 (E. coli)
OCTS3NTH1nth (E.coli endonuclease III)-like 1
NTH1 (endonuclease III-like protein 1 or NTHL1) has both apurinic/apyrimidinic endonuclease activity and a DNA N-glycosylase activity. The C-O-P bond 3' to apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. NTH1 associates with 1 4Fe-4S cluster and this cluster has been proposed to involve in proper positioning of enzyme along the DNA strand. With potential for incising damaged DNA at cytosines, thymines and guanines, NTH1 acts on a damaged strand 5' from the damaged site, for playing essential role in repair of both oxidative DNA damage and spontaneous mutagenic lesions. Localized in the nucleus, NTH1 is widely expressed with highest levels in heart and its expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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