NTH1 Antibody

Images

 
Western Blot: NTH1 Antibody [NB100-302] - Analysis of A549 cells using NB100-302. A band is seen at 35 kDa representing NTH1.
Immunocytochemistry/ Immunofluorescence: NTH1 Antibody [NB100-302] - The NTH1 antibody was tested in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NTH1 Antibody Summary

Immunogen
Full-length recombinant human NTH1. [UniProt# P78549]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NTHL1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This NTH1 antibody is useful for Western blot, where a band at ~37 kD. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB100-302.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for NTH1 Antibody

  • EC 4.2.99.18
  • endonuclease III-like protein 1
  • nth endonuclease III-like 1 (E. coli)
  • NTH1
  • NTHL1
  • OCTS3
  • OCTS3NTH1nth (E.coli endonuclease III)-like 1

Background

NTH1 (endonuclease III-like protein 1 or NTHL1) has both apurinic/apyrimidinic endonuclease activity and a DNA N-glycosylase activity. The C-O-P bond 3' to apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. NTH1 associates with 1 4Fe-4S cluster and this cluster has been proposed to involve in proper positioning of enzyme along the DNA strand. With potential for incising damaged DNA at cytosines, thymines and guanines, NTH1 acts on a damaged strand 5' from the damaged site, for playing essential role in repair of both oxidative DNA damage and spontaneous mutagenic lesions. Localized in the nucleus, NTH1 is widely expressed with highest levels in heart and its expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NTH1 Antibody (NB100-302)(1)

We have publications tested in 1 confirmed species: Human.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NTH1 Antibody (NB100-302). (Showing 1 - 1 of 1 FAQ).

  1. How much immunogen in mass did you use to get this result? What's your secondary detection approach?
    • Please see our full Western blot protocol at this link. NB7183 is the secondary antibody we use and we loaded 40 ug of protein in the gel.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NTHL1
Entrez
Uniprot