NTH1 Antibody - BSA Free

Western Blot: NTH1 Antibody [NB100-302] - Analysis of A549 cells using NB100-302. A band is seen at 35 kDa representing NTH1.

Immunocytochemistry/Immunofluorescence: NTH1 Antibody [NB100-302] - The NTH1 antibody was tested in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NTH1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NTH1 Antibody - BSA Free (NB100-302) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-NTH1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length recombinant human NTH1. [UniProt# P78549]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NTHL1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Western Blot 1:1000-1:5000
• Application Notes: In Western blot, a band is seen at ~37 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for NTH1 Antibody - BSA Free

• EC 4.2.99.18
• endonuclease III-like protein 1
• nth endonuclease III-like 1 (E. coli)
• NTH1
• NTHL1
• OCTS3
• OCTS3NTH1nth (E.coli endonuclease III)-like 1

Background

NTH1 (endonuclease III-like protein 1 or NTHL1) has both apurinic/apyrimidinic endonuclease activity and a DNA N-glycosylase activity. The C-O-P bond 3' to apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. NTH1 associates with 1 4Fe-4S cluster and this cluster has been proposed to involve in proper positioning of enzyme along the DNA strand. With potential for incising damaged DNA at cytosines, thymines and guanines, NTH1 acts on a damaged strand 5' from the damaged site, for playing essential role in repair of both oxidative DNA damage and spontaneous mutagenic lesions. Localized in the nucleus, NTH1 is widely expressed with highest levels in heart and its expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NTH1 Antibody (NB100-302)(1)

Publication using NB100-302

• Odell ID, Newick K, Heintz NH et al. Non-specific DNA binding interferes with the efficient excision of oxidative lesions from chromatin by the human DNA glycosylase, NEIL1. DNA Repair (Amst) 2010-02-04 [PMID: 20005182] (Human)

FAQs for NTH1 Antibody (NB100-302). (Showing 1 - 1 of 1 FAQ).

1. How much immunogen in mass did you use to get this result? What's your secondary detection approach?
   • Please see our full Western blot protocol at this link. NB7183 is the secondary antibody we use and we loaded 40 ug of protein in the gel.

Bioinformatics

• Gene Symbol: NTHL1
• Entrez:
  • 4913(Human)
• Uniprot:
  • P78549()