NTH1 Antibody (S08-8E9)

Western Blot: NTH1 Antibody (S08-8E9) [NBP3-19722] - Western blot detection of NTH1 in K562, Hela cell lysates using NBP3-19722 (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S08-8E9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

NTH1 Antibody (S08-8E9) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human NTH1 (Uniprot # P78549)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NTHL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for NTH1 Antibody (S08-8E9)

• EC 4.2.99.18
• endonuclease III-like protein 1
• nth endonuclease III-like 1 (E. coli)
• NTH1
• NTHL1
• OCTS3
• OCTS3NTH1nth (E.coli endonuclease III)-like 1

Background

Oxygen radicals damage chromosonal DNA causing cell death and inducing mutations. Although a major focus of oxidatively damaged DNA has centered on the repair of 8-oxo-G, a number of other damaged DNA sites are created by free-radical attack on DNA. The human NTH1 repair protein is one enzyme that has been shown to act on a large number of these other oxidatively damaged DNA sites. A homologue of E. coli Endonuclease III, NTH is a DNA glycosylase with apurinic/apyrimidinic lyase activity. The NTH protein has broad substrate specificity, including numerous ring saturation and fragmentation products of pyrimidines. Research indicates NTH plays a crucial role in removal of oxidative base lesions in mitochondrial DNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NTHL1