NTH1 Antibody (22H10) - BSA Free

Immunohistochemistry: NTH1 Antibody (22H10) [NBP3-26456] - Image of NTH1 Antibody (22H10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot: NTH1 Antibody (22H10) [NBP3-26456] - Positive Western Blot detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, PC-3 whole cell lysate. All lanes: NTH1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 35, 34, 33 kDa Observed band size: 35 kDa

Immunocytochemistry/Immunofluorescence: NTH1 Antibody (22H10) [NBP3-26456] - Staining of PC-3 cell with NTH1 Antibody (22H10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 604-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: NTH1 Antibody (22H10) [NBP3-26456] - Overlay Peak curve showing HepG2 cells stained with NTH1 Antibody (22H10) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 22H10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NTH1 Antibody (22H10) - BSA Free Summary

• Description: Novus Biologicals Rabbit NTH1 Antibody (22H10) - BSA Free (NBP3-26456) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human NTH1 [UniProt P78549]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NTHL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NTH1 Antibody (22H10) - BSA Free

• EC 4.2.99.18
• endonuclease III-like protein 1
• nth endonuclease III-like 1 (E. coli)
• NTH1
• NTHL1
• OCTS3
• OCTS3NTH1nth (E.coli endonuclease III)-like 1

Background

Oxygen radicals damage chromosonal DNA causing cell death and inducing mutations. Although a major focus of oxidatively damaged DNA has centered on the repair of 8-oxo-G, a number of other damaged DNA sites are created by free-radical attack on DNA. The human NTH1 repair protein is one enzyme that has been shown to act on a large number of these other oxidatively damaged DNA sites. A homologue of E. coli Endonuclease III, NTH is a DNA glycosylase with apurinic/apyrimidinic lyase activity. The NTH protein has broad substrate specificity, including numerous ring saturation and fragmentation products of pyrimidines. Research indicates NTH plays a crucial role in removal of oxidative base lesions in mitochondrial DNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NTHL1