Recombinant Human NT5M Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human NT5M Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 32-228 of Human NT5M

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
NT5M
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
25.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human NT5M Protein

  • 3'-nucleotidase, mitochondrialDNT2
  • 5' nucleotidase, mitochondrial
  • 5'
  • 5(3)-deoxyribonucleotidase
  • Deoxy-5'-nucleotidase 2
  • dNT2
  • dNT-25'(3')-deoxyribonucleotidase, mitochondrial
  • EC 3.1.3
  • EC 3.1.3.-
  • mdN
  • mitochondrial 5' nucleotidase

Background

NT5M, also known as DNT2, belongs to the 5'(3')-deoxyribonucleotidase family. It localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides. The gene is located on chromosome 17 p11.2 in the critical region deleted in the Smith-Magenis syndrome (SMS), a genetic disease of unknown etiology. Recombinant human NT5M protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol NT5M