NSF Antibody - Azide Free

Western Blot: NSF Antibody [NB300-733]

• Reactivity: Rt, Hu, Mu, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

NSF Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit NSF Antibody - Azide Free (NB300-733) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # Q9QUL6
• Localization: Cytoplasmic
• Specificity: Specific for endogenous levels of the ~75 kDa NSF protein.
• Predicted Species: Human (100%), Mouse (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NSF
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NSF Overexpression Lysate

Reactivity Notes

It is anticipated that the antibody will also work with human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for NSF Antibody - Azide Free

• EC 3.6.4.6
• NEM-sensitive fusion protein
• N-ethylmaleimide-sensitive factor
• N-ethylmaleimide-sensitive factor-like protein
• N-ethylmaleimide-sensitive fusion protein
• SKD2
• vesicle-fusing ATPase
• Vesicular-fusion protein NSF

Background

NSF is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NSF
• Entrez:
  • 4905(Human)
  • 18195(Mouse)
  • 60355(Rat)
• Uniprot:
  • Q9QUL6(Rat)