Nrf2 Antibody (383727) [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-), treated (+) or mock treated (m) with 10 µM MG132 (Catalog # 1748) for 4 hours and 10 pmol siRNA. PVDF membrane was probed with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
383727
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Nrf2 Antibody (383727) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Nrf2
Met17-Asn605
Accession # Q16236
Specificity
Detects human Nrf2 in Western blots. In Western blots, no cross-reactivity with mouse and rat Nrf2 and human Nrf1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NFE2L2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4
using
MAB3925 in the following application:

Publications
Read Publications using
MAB3925 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nrf2 Antibody (383727) [Unconjugated]

  • HEBP1
  • NFE2L2
  • NF-E2-related factor 2
  • NFE2-related factor 2
  • NRF2 erythroid derived 2, like 2
  • Nrf2
  • nuclear factor (erythroid-derived 2)-like 2
  • nuclear factor erythroid 2-related factor 2
  • nuclear factor erythroid-derived 2-like 2

Background

NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nrf2 Antibody (MAB3925)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(5)
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Filter By Species
Human
(5)
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Showing Publications 1 - 5 of 5.
Publications using MAB3925 Applications Species
Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??. Molecular Medicine Reports, 2018;0(0):1791-3004. 2018 [PMID: 29956764] (Western Blot, Human) Western Blot Human
B Liu, L Xu, X Yu, W Li, X Sun, S Xiao, M Guo, H Wang Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF?? Mol Med Rep, 2018;18(2):1987-1994. 2018 [PMID: 29956764] (Western Blot, Human) Western Blot Human
G Kontostath, J Zoidakis, M Makridakis, V Lygirou, G Mermelekas, T Papadopoul, K Vougas, A Vlamis-Gar, P Drakakis, D Loutradis, A Vlahou, NP Anagnou, KI Pappa Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis Biomed Res Int, 2017;2017(0):4180703. 2017 [PMID: 28261610] (Western Blot, Human) Western Blot Human
Vitiligo-inducing phenols activate the unfolded protein response in melanocytes resulting in upregulation of IL6 and IL8. J. Invest. Dermatol., 2012;132(11):2601-9. 2012 [PMID: 22696056] (Western Blot, Human) Western Blot Human
Cross SA, Cook DR, Chi AW, Vance PJ, Kolson LL, Wong BJ, Jordan-Sciutto KL, Kolson DL Dimethyl fumarate, an immune modulator and inducer of the antioxidant response, suppresses HIV replication and macrophage-mediated neurotoxicity: a novel candidate for HIV neuroprotection. J. Immunol., 2011;187(10):5015-25. 2011 [PMID: 21976775] (Western Blot, Human) Western Blot Human

Reviews for Nrf2 Antibody (MAB3925) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using MAB3925:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Nrf2 MAB3925
Enlarge
3
reviewed by:
YONGCHUL JANG
WB Mouse 09/29/2017
View

Summary

ApplicationWestern Blot
Sample TestedBrain (substantia nigra)
SpeciesMouse
LotMAB3925
  5
reviewed by:
Anonymous
WB Mouse 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 19282378
SpeciesMouse

Blocking

Blocking DetailsSee PMID 19282378

Details

Detection NotesSee PMID 19282378

Comments

CommentsIndependent review from antibodyresource.com
  4
reviewed by:
Anonymous
WB Human 02/20/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21976775
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21976775

Details

Detection NotesSee PMID 21976775

Comments

CommentsIndependent review from antibodyresource.com

Product General Protocols

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FAQs for Nrf2 Antibody (MAB3925) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Nrf2.


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PGC-1 alpha: Roles in Mitochondrial Biogenesis and Disease
An important aspect of mitochondria maintenance includes biogenesis to replenish damaged and degraded mitochondrial structures. The regulation of mitochondrial biogenesis is very complex and numerous genes regulate and synchronize protein synthesis fr...  Read full blog post.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

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Recent Reviews

4
5
1
4
1
3
1
2
0
1
0

YONGCHUL JANG
09/29/2017
Application: WB
Species: Mouse

 
Anonymous
02/20/2015
Application: WB
Species: Mouse

 
Anonymous
02/20/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol NFE2L2
Uniprot