NRBP1 Antibody (OTI5C3)

Western Blot: NRBP1 Antibody (OTI5C3) [NBP1-47883] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NRBP1 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: NRBP1 Antibody (OTI5C3) [NBP1-47883] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NRBP1.

Immunohistochemistry-Paraffin: NRBP1 Antibody (OTI5C3) [NBP1-47883] - Staining of paraffin-embedded Human pancreas tissue using anti-NRBP1 mouse monoclonal antibody.

Flow Cytometry: NRBP1 Antibody (OTI5C3) [NBP1-47883] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NRBP1 antibody, and then analyzed by flow cytometry.

Western Blot: NRBP1 Antibody (OTI5C3) [NBP1-47883] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1.

Immunohistochemistry-Paraffin: NRBP1 Antibody (OTI5C3) [NBP1-47883] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NRBP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NRBP1 Antibody (OTI5C3) [NBP1-47883] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NRBP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NRBP1 Antibody (OTI5C3) [NBP1-47883] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NRBP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NRBP1 Antibody (OTI5C3) [NBP1-47883] - Staining of paraffin-embedded Human breast tissue using anti-NRBP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NRBP1 Antibody (OTI5C3) [NBP1-47883] - Staining of paraffin-embedded Human colon tissue using anti-NRBP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NRBP1 Antibody (OTI5C3) [NBP1-47883] - Staining of paraffin-embedded Human lymphoma tissue using anti-NRBP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NRBP1 Antibody (OTI5C3) [NBP1-47883] - Staining of paraffin-embedded Human Ovary tissue using anti-NRBP1 mouse monoclonal antibody.

Immunoprecipitation: NRBP1 Antibody (OTI5C3) [NBP1-47883] - (Negative control: IP without adding anti-NRBP1 antibody.). For each experiment, 500ul of DDK tagged NRBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NRBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: OTI5C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.48 mg/ml

NRBP1 Antibody (OTI5C3) Summary

• Description: Novus Biologicals Mouse NRBP1 Antibody (OTI5C3) (NBP1-47883) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length protein expressed in 293T cell transfected with human NRBP1 expression vector
• Specificity: This antibody is specific for Homo sapiens nuclear receptor binding protein 1 (NRBP1).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NRBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:1000-2000
• Theoretical MW: 59.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.48 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NRBP1 Antibody (OTI5C3)

• BCON3NRBPMADM
• FLJ27109
• FLJ35541
• MUDPNP
• multiple domain putative nuclear protein
• myeloid leukemia factor 1 adaptor molecule
• nuclear receptor binding protein 1
• nuclear receptor binding protein
• nuclear receptor-binding protein

Background

May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NRBP1
• Entrez:
  • 29959(Human)