NRBP1 Antibody (4D2) - Azide and BSA Free

Western Blot: NRBP1 Antibody (4D2) [H00029959-M01] - NRBP monoclonal antibody (M01), clone 4D2 Analysis of NRBP expression in HL-60.

Immunocytochemistry/Immunofluorescence: NRBP1 Antibody (4D2) [H00029959-M01] - Analysis of monoclonal antibody to NRBP1 on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: NRBP1 Antibody (4D2) [H00029959-M01] - Detection limit for recombinant GST tagged NRBP1 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NRBP1 Antibody (4D2) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse NRBP1 Antibody (4D2) - Azide and BSA Free (H00029959-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-NRBP1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: NRBP1 (AAH01221, 436 a.a. ~ 535 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PAEVETRKVVLMQCNIESVEEGVKHHLTLLLKLEDKLNRHLSCDLMPNENIPELAAELVQLGFISEADQSRLTSLLEETLNKFNFARNSTLNSAAVTVSS
• Specificity: NRBP1 - nuclear receptor binding protein
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NRBP1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NRBP1 Antibody (4D2) - Azide and BSA Free

• BCON3NRBPMADM
• FLJ27109
• FLJ35541
• MUDPNP
• multiple domain putative nuclear protein
• myeloid leukemia factor 1 adaptor molecule
• nuclear receptor binding protein 1
• nuclear receptor binding protein
• nuclear receptor-binding protein

Background

NRBP1 may play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NRBP1 Antibody (H00029959-M01)(4)

Publications using H00029959-M01

• Linghui Q, Xiaosong S, Fei Z et al. CircLRP6 contributes to prostate cancer growth and metastasis by binding to miR-330-5p to up-regulate NRBP1. World J Surg Oncol. 2021-06-22 [PMID: 34158077]
• Yasukawa T, Tsutsui A, Tomomori-Sato C et al. NRBP1-Containing CRL2/CRL4A Regulates Amyloid ? Production by Targeting BRI2 and BRI3 for Degradation. Cell Rep. 2020-03-10 [PMID: 32160551]
• Wu Q, Zhou X, Li P et al. High NRBP1 expression promotes proliferation and correlates with poor prognosis in bladder cancer. J Cancer. 2019-07-10 [PMID: 31413746]
• Liao Y, Yang Z, Huang J et al. Nuclear receptor binding protein 1 correlates with better prognosis and induces caspase-dependent intrinsic apoptosis through the JNK signalling pathway in colorectal cancer. Cell Death Dis 2018-03-22 [PMID: 29567997]

Bioinformatics

• Gene Symbol: NRBP1
• Entrez:
  • 29959(Human)
• OMIM:
  • 606010(Human)
• Uniprot:
  • AAH01221(Human)