NRAGE/MAGED1 Antibody (1E1) - Azide and BSA Free

Western Blot: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06] - Analysis of MAGED1 expression in Jurkat (Cat # L017V1).

Immunohistochemistry-Paraffin: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06] - Analysis of monoclonal antibody to MAGED1 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml

ELISA: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06] - Detection limit for recombinant GST tagged MAGED1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NRAGE/MAGED1 Antibody (1E1) - Azide and BSA Free Summary

• Immunogen: MAGED1 (NP_001005333, 117 a.a. ~ 226 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EMADIQVSAAAARPKSAFKVQNATTKGPNGVYDFSQAHNAKDVPNTQPKAAFKSQNATPKGPNAAYDFSQAATTGELAANKSEMAFKAQNATTKVGPNATYNFSQSLNAN
• Specificity: MAGED1 - melanoma antigen family D, 1 (1E1)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAGED1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NRAGE/MAGED1 Antibody (1E1) - Azide and BSA Free

• Dlxin-1
• MAGE tumor antigen CCF
• MAGED1
• melanoma antigen family D, 1
• melanoma-associated antigen D1
• Neurotrophin receptor-interacting MAGE homolog
• NRAGE MAGE-D1 antigen
• NRAGE

Background

This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAGED1
• Entrez:
  • 9500(Human)
• Uniprot:
  • NP_001005333(Human)