NPY5R Antibody

Product Discontinued

NPY5R Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 230-286 of Human NPY5-R.
• Specificity: NPY5R (M264) detects endogenous levels of NPY5-R protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPY5R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of NPY5-R pAb in extracts from COLO/Jurkat/HepG2 cells.
  Immunohistochemistry (IHC) analyzes of NPY5R pAb in paraffin-embedded human breast carcinoma tissue.
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NPY5R Antibody

• neuropeptide Y receptor type 5
• neuropeptide Y receptor Y5
• NPY5R
• NPYR5NPY5-R
• NPY-Y5 receptor
• NPYY5-R
• Y5 receptor

Background

Pancreatic polypeptide (PP), neuropeptide Y (NPY), and peptide YY (PYY) are related 36-amino acid hormones. A number of structurally related receptors for these peptides have been isolated, NPY1-R, NPY2-R, NPY3-R, NPY4-R, NPY5R, and NPY6-R. NPY4-R is expressed in several human tissues, including brain, coronary artery, and ileum. NPY4-R maps to human chromosome 10q11.2-q21.2. NPY-5R, isolated from rat hypothalamus, encodes a 456-amino acid protein with less than 35% overall identity to known Y-type receptors. The human NPY5R sequence is nearly identical to, but in the opposite orientation from, that of the human NPY-1R sequence. NPY5R localizes to the paraventricular hypothalamic nucleus, the lateral hypothalamus, and other locations consistent with a role in the control of feeding behavior. The gene which encodes NPY5R maps to human chromosome 4q31-32. NPY-6R is abundantly expressed in human heart and skeletal muscle and the gene which encodes NPY-6R maps to human chromosome 5q31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NPY5R Antibody (NBP1-00957)(2)

Publications using NBP1-00957

• Czarnecka M, Trinh E, Lu C et al. Neuropeptide Y receptor Y5 as an inducible pro-survival factor in neuroblastoma: implications for tumor chemoresistance. Oncogene. 2014-08-18 [PMID: 25132261] (IHC-P, Human)
• Adnani MT The Role of Neuropeptide Y and NPY5R in Ewing Sarcoma Metastasis Thesis 2020-01-01 (IHC-P, Mouse)

FAQs for NPY5R Antibody (NBP1-00957). (Showing 1 - 2 of 2 FAQs).

1. I have used your Y5R antibody for IHC. It works well. To show the specificity of Y5R labeling, is it possible to block the labeling with the peptide or Y5R protein pre-treatment to the antibody? The catalog number is NBPI-00957.
   • Unfortunately we do not have the blocking peptide for that one available to run that negative control competition assay.
2. Which cell lysate should I use with Anti-human Neuropeptide Y5R (cat # NBP1-00957)?
   • NPY5R: Brain; hypothalamus.

Bioinformatics

• Gene Symbol: NPY5R
• Entrez:
  • 4889(Human)
  • 18168(Mouse)
  • 25340(Rat)
• Uniprot:
  • Q15761(Human)