NPY5R Antibody

Images

 
Western Blot: NPY5R Antibody [NBP1-00957] - Lane1:HEK293T whole cell lysate. Lane2:Raw264.7 whole cell lysate. Lane3:H9C2 whole cell lysate.
Immunohistochemistry: NPY5R Antibody [NBP1-00957] - Immunohistochemistry (IHC) analyzes of NPY5R (M264) antibody in paraffin-embedded human breast carcinoma tissue
Western Blot: NPY5R Antibody [NBP1-00957] - Western blot (WB) analysis of NPY5R (M264) antibody in extracts from COLO/Jurkat/HepG2 cells.

Product Details

Summary
Product Discontinued
View other related NPY5R Primary Antibodies

Order Details


    • Catalog Number
      NBP1-00957
    • Availability
      Product Discontinued

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NPY5R Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 230-286 of Human NPY5-R.
Specificity
NPY5R (M264) detects endogenous levels of NPY5-R protein.
Clonality
Polyclonal
Host
Rabbit
Gene
NPY5R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of NPY5-R pAb in extracts from COLO/Jurkat/HepG2 cells.
Immunohistochemistry (IHC) analyzes of NPY5R pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-00957 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NPY5R Antibody

  • neuropeptide Y receptor type 5
  • neuropeptide Y receptor Y5
  • NPY5R
  • NPYR5NPY5-R
  • NPY-Y5 receptor
  • NPYY5-R
  • Y5 receptor

Background

Pancreatic polypeptide (PP), neuropeptide Y (NPY), and peptide YY (PYY) are related 36-amino acid hormones. A number of structurally related receptors for these peptides have been isolated, NPY1-R, NPY2-R, NPY3-R, NPY4-R, NPY5R, and NPY6-R. NPY4-R is expressed in several human tissues, including brain, coronary artery, and ileum. NPY4-R maps to human chromosome 10q11.2-q21.2. NPY-5R, isolated from rat hypothalamus, encodes a 456-amino acid protein with less than 35% overall identity to known Y-type receptors. The human NPY5R sequence is nearly identical to, but in the opposite orientation from, that of the human NPY-1R sequence. NPY5R localizes to the paraventricular hypothalamic nucleus, the lateral hypothalamus, and other locations consistent with a role in the control of feeding behavior. The gene which encodes NPY5R maps to human chromosome 4q31-32. NPY-6R is abundantly expressed in human heart and skeletal muscle and the gene which encodes NPY-6R maps to human chromosome 5q31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NPY5R Antibody (NBP1-00957)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NPY5R Antibody (NBP1-00957). (Showing 1 - 2 of 2 FAQ).

  1. I have used your Y5R antibody for IHC. It works well. To show the specificity of Y5R labeling, is it possible to block the labeling with the peptide or Y5R protein pre-treatment to the antibody? The catalog number is NBPI-00957.
    • Unfortunately we do not have the blocking peptide for that one available to run that negative control competition assay.
  2. Which cell lysate should I use with Anti-human Neuropeptide Y5R (cat # NBP1-00957)?
    • NPY5R: Brain; hypothalamus.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol NPY5R
Entrez
Uniprot