NPM1 Antibody

Western Blot: NPM1 Antibody [NBP2-27157] - Analysis of Nucleophosmin in human spleen tissue lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse spleen using NBP2-27204. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: NPM1 Antibody [NBP2-27157] - Analysis of Nucleophosmin in formalin-fixed, paraffin-embedded human liver tissue using this antibody at 5 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NPM1 Antibody Summary

• Immunogen: Within the range of amino acids 21-53 of human Nucleophosmin were used as the immunogen.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Western Blot 2-5ug/ml~

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for NPM1 Antibody

• B23
• MGC104254
• NPM1
• NPMnucleophosmin/nucleoplasmin family, member 1
• Nucleolar phosphoprotein B23
• Nucleolar protein NO38
• nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Nucleophosmin
• Numatrin

Background

Nucleophosmin is a major nucleolar multifunctional phosphoprotein involved in diverse cellular processes such as protein chaperoning, histone assembly cell-cycle progression, apoptosis, cell differentiation, and regulation of tumor suppressors like TP53/p53 and ARF. It shuttles between the nucleus and cytoplasm during the cell cycle. Reports suggested Nucleophosmin is phosphorylated mainly at its central acidic domain by several upstream kinases and its phosphorylation appears to be involved in regulating its functions in ribosome biogenesis and centrosome duplication. It is also reported to act as sensor of cellular stress thus acting as alarmin. Recent reports suggested that hyperactive Cdk2 and Cdk4 deregulate the licensing of the centrosome duplication cycle in p53-null cells by hyperphosphorylating nucleophosmin (NPM) at Thr199.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NPM1