NPM1 Antibody (7H10B9)

Western Blot: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.

Immunocytochemistry/Immunofluorescence: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Immunohistochemistry-Paraffin: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis of human liver carcinoma tissues, showing nuclear localization using NPM mouse mAb with DAB staining.

• Reactivity: Hu, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 7H10B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NPM1 Antibody (7H10B9) Summary

• Immunogen: Purified recombinant fragment of human NPM (2-265) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPM1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for NPM1 Antibody (7H10B9)

• B23
• MGC104254
• NPM1
• NPMnucleophosmin/nucleoplasmin family, member 1
• Nucleolar phosphoprotein B23
• Nucleolar protein NO38
• nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Nucleophosmin
• Numatrin

Background

Nucleophosmin (NPM) is a multi-functional protein that is involved in many cellular activities including cell growth, proliferation and transformation. It is frequently overexpressed in solid tumors, and has recently been found to be mutated and aberrantly localized in the cytoplasm of leukaemic blasts in patients with acute myeloid leukemia (AML).

NMP antibodies are useful tools as tumor markers in cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NPM1 Antibody (NBP1-47354)(2)

Publications using NBP1-47354

• Glushonkov O, Real E, Boutant E et al. Optimized protocol for combined PALM-dSTORM imaging Sci Rep 2018-06-08 [PMID: 29884886] (ICC/IF, Human)
• Morgado-Palacin L, Llanos S, Urbano M et al. Non-genotoxic activation of p53 through the RPL11-dependent ribosomal stress pathway. Carcinogenesis. 2014-10-24 [PMID: 25344835] (WB)

Bioinformatics

• Gene Symbol: NPM1
• Entrez:
  • 4869(Human)