NPM1 Antibody (7H10B9)

Images

 
Western Blot: NPM1 Antibody (7H10B9) [NBP1-47354] - Western blot analysis using anti-Acetyl-Histone H3 (Lys9) pAb against NIH/3T3 cell lysate, untreated (1), TSA-treated (2) (to induce acetylation).
Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Immunohistochemistry-Paraffin: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.
Immunohistochemistry-Paraffin: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis of human liver carcinoma tissues, showing nuclear localization using NPM mouse mAb with DAB staining.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
7H10B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NPM1 Antibody (7H10B9) Summary

Immunogen
Purified recombinant fragment (aa2-265) of human NPM expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NPM1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Application Notes
This antibody is useful in Western Blot, Immunohistochemistry-Paraffin,Immunofluorescence, ELISA. Although not tested this antibody may be useful in Immunohistochemistry-Frozen.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-47354.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for NPM1 Antibody (7H10B9)

  • B23
  • MGC104254
  • NPM1
  • NPMnucleophosmin/nucleoplasmin family, member 1
  • Nucleolar phosphoprotein B23
  • Nucleolar protein NO38
  • nucleophosmin (nucleolar phosphoprotein B23, numatrin)
  • Nucleophosmin
  • Numatrin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NPM1 Antibody (NBP1-47354)(1)

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NPM1
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