Home » NPM1 » NPM1 Antibodies » NPM1 Antibody (7H10B9)

NPM1 Antibody (7H10B9)

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.
Western Blot: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Immunohistochemistry-Paraffin: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis of human liver carcinoma tissues, showing nuclear localization using NPM mouse mAb with DAB staining.
Western Blot: NPM1 Antibody (7H10B9) [NBP1-47354] - Western blot analysis using anti-Acetyl-Histone H3 (Lys9) pAb against NIH/3T3 cell lysate, untreated (1), TSA-treated (2) (to induce acetylation).

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
7H10B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

NPM1 Antibody (7H10B9) Summary

Immunogen
Purified recombinant fragment (aa2-265) of human NPM expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NPM1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Application Notes
This antibody is useful in Western Blot, Immunohistochemistry-Paraffin,Immunofluorescence, ELISA. Although not tested this antibody may be useful in Immunohistochemistry-Frozen.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-47354.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for NPM1 Antibody (7H10B9)

  • B23
  • MGC104254
  • NPM1
  • NPMnucleophosmin/nucleoplasmin family, member 1
  • Nucleolar phosphoprotein B23
  • Nucleolar protein NO38
  • nucleophosmin (nucleolar phosphoprotein B23, numatrin)
  • Nucleophosmin
  • Numatrin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Flt-3/Flk-2/CD135
Antibody (7E8.2C8)
NBP2-42210
Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.
Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells while the tumor stroma was largely negative for the staining.
Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis
Species: Hu
Applications: WB, Flow, IHC, IHC-P
ALK/CD246 Antibody
AF4210
Western blot shows lysates of Rh30 human rhabdomyosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human ALK/CD246 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4210) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for ALK/CD246 at approximately 220 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ALK/CD246 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Sheep Anti-Human ALK/CD246 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4210) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu
Applications: WB, IHC
Nucleolin Antibody
NB600-241
Western Blot: Nucleolin Antibody [NB600-241] - Detection of nucleolin in crude PD31 nuclear extracts.
Immunocytochemistry: Nucleolin Antibody [NB600-241] - Human prostate epithelial cells stained with anti-nucleolin (red), CFSE (green) retaining cell has increased nucleolin in nucleolus. DAPI (blue). This image was submitted via customer Review.
Immunohistochemistry: Nucleolin Antibody [NB600-241] - Human prostate epithelial cells stained with anti-nucleolin (red), CFSE (green) retaining cell has increased nucleolin in nucleolus. DAPI (blue). Verified customer review.
(2 Reviews)
(17 Publications)
Species: Hu, Mu, Rt, Pl
Applications: WB, Simple Western, ChIP, EM, ICC/IF, IHC, IHC-P, IM, ICC
p19ARF Antibody
NB200-106
Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.
Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
Immunohistochemistry-Paraffin: p19ARF Antibody [NB200-106] - IHC analysis of formalin fixed paraffin-embedded (FFPE) mouse spleen using p19ARF antibody at 10 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining in the germinal centers of spleen was observed. Staining was performed by Histowiz.
(1 Review)
(40 Publications)
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(2 Reviews)
(22 Publications)
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
CEBP alpha
Antibody (15C8)
NB600-1438
Western Blot: CEBP alpha Antibody (15C8) [NB600-1438] - Analysis of transfected 293T cells.
Flow Cytometry: CEBP alpha Antibody (15C8) [NB600-1438] - Flow cytometry analysis of CEBP alpha was done on THP-1 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with CEBP alpha Mouse Monoclonal Antibody (MA1825, red histogram) or with mouse isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor (R) 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune (R) Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IP
TPT1/TCTP Antibody
NBP2-41206
Western Blot: TPT1/TCTP Antibody [NBP2-41206] - Western blot analysis of TPT1 in human brain tissue lysate with TPT1 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry: TPT1/TCTP Antibody [NBP2-41206] - Immunohistochemistry of TPT1 in rat brain tissue with TPT1 antibody at 2.5 ug/ml.
Immunofluorescence: TPT1/TCTP Antibody [NBP2-41206] - TPT1 in rat brain tissue with TPT1 antibody at 20 ug/ml.
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF
CDK5 Activator 1
Antibody (739304)
MAB7357
Western blot shows lysates of human brain (cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CDK5 Activator 1 Monoclonal Antibody (Catalog # MAB7357) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CDK5 Activator 1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
Species: Hu
Applications: WB
Tmp21/p23 Antibody
NB110-57586
Western Blot: TMP21 Antibody [NB110-57586] - Chicken embryo extract - Data supplied by Dr Yonghua Zhang of McGill University.
Immunohistochemistry: TMP21 Antibody [NB110-57586] - Detection of TMP21 in central neurons.
Western Blot: TMP21 Antibody [NB110-57586] - Detection of TMP21 in human liver lysate.
Species: Hu, Mu, Rt, Bv, Ch, Pm, Rb
Applications: WB, IHC, IHC-P
RRAS Antibody
NB100-56161
RRAS Antibody
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
p23/PTGES3
Antibody (JJ3)
NB300-576
Western Blot: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of 25 ug of Hela (Lane 1), HepG2 (Lane 2), and mouse spleen cell lysates (Lane 3) and a molecular weight protein ladder.
Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 at a dilution of 1:500 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Normal biopsies of deparaffinized Human testis tissue.
(4 Publications)
Species: Hu, Mu, Rt, Ch, GP, Pm, Rb, Xp
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Fibrillarin
Antibody (38F3)
NB300-269
Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - of whole yeast protein extracts with a collection of our antibodies. The blot for Fibrillarin Antibody (38F3) is in the indicated lane, and the number indicates the SDS-PAGE molecular weight in kiloDaltons.
Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - IF Confocal analysis of MCF7 cells using Fibrillarin antibody (NB300-269, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry-Paraffin: Fibrillarin Antibody (38F3) [NB300-269] - IHC staining of Fibrillarin in human ovarian cancer using DAB with hematoxylin counterstain.
(2 Reviews)
(11 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ch, Dr, Eq, Ye
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
CD30/TNFRSF8
Antibody (OTI1C6)
NBP2-46157
Western Blot: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8.
Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human endometrium tissue.
Flow Cytometry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - (Blue).(1:100)
(1 Review)
Species: Hu
Applications: WB, Flow, IHC, IHC-P
MID1 Antibody
NBP1-26612
Western Blot: MID1 Antibody [NBP1-26612] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 1 ug/ml.
(1 Publication)
Species: Hu
Applications: IP (-), WB
CD34 Antibody (MEC
14.7)
NB600-1071
Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.
Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
(17 Publications)
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Isocitrate
Dehydrogenase 1/IDH1 ...
NBP1-47804
Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Isocitrate dehydrogenasemonoclonal antibody.
Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Isocitrate dehydrogenase.
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Endometrium tissue.
(1 Review)
Species: Hu, Mk
Applications: WB, ICC/IF, IHC, IHC-P
KMT2A/MLL Antibody
NB600-248
Western Blot: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear extract (50 and 15 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-248 used for IP at 6 ug per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 antibody NB600-249. For blotting immunoprecipitated MLL1, NB600-248 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
(1 Review)
(25 Publications)
Species: Hu
Applications: WB, IP
WT1 Antibody
(6F-H2)
NB110-60011
Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Whole cell protein from human K562, Hek293 and kidney tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WT1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry: WT1 Antibody (6F-H2) [NB110-60011] - IHC analysis of Wilms Tumor 1 in human renal cancer using DAB with hematoxylin counterstain.
Simple Western: WT1 Antibody (6F-H2) [NB110-60011] - Simple Western lane view shows a specific band for WT1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(1 Review)
(4 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P, IP
CDK2 Antibody (1A6)
NBP2-37507
Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Western blot analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.
Immunocytochemistry/Immunofluorescence: CDK2 Antibody (1A6) [NBP2-37507] - Immunofluorescence analysis of HeLa cells using CDK2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: CDK2 Antibody (1A6) [NBP2-37507] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CDK2 mouse mAb with DAB staining.
(1 Review)
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NPM1 Antibody
(7H10B9)
NBP1-47354
NPM1 Antibody (7H10B9)
Species: Hu, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for NPM1 Antibody (NBP1-47354)(1)

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
All Species

Reviews for NPM1 Antibody (NBP1-47354) (0)

There are no reviews for NPM1 Antibody (NBP1-47354). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NPM1 Antibody (NBP1-47354) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional NPM1 Products

Bioinformatics Tool for NPM1 Antibody (NBP1-47354)

Discover related pathways, diseases and genes to NPM1 Antibody (NBP1-47354). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NPM1 Antibody (NBP1-47354)

Discover more about diseases related to NPM1 Antibody (NBP1-47354).
 

Pathways for NPM1 Antibody (NBP1-47354)

View related products by pathway.

PTMs for NPM1 Antibody (NBP1-47354)

Learn more about PTMs related to NPM1 Antibody (NBP1-47354).

Blogs on NPM1

There are no specific blogs for NPM1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NPM1 Antibody (7H10B9) and receive a gift card or discount.


Bioinformatics

Gene Symbol NPM1
Entrez