Npas4 Antibody (S408-79) - BSA Free

Western Blot: Npas4 Antibody (S408-79) [NBP2-59332] - Western Blot analysis of Rat Brain showing detection of ~90 kDa Npas4 protein using Mouse Anti-Npas4 Monoclonal Antibody, Clone S408-79 (NBP2-59332). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Npas4 Monoclonal Antibody (NBP2-59332) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

Immunocytochemistry/Immunofluorescence: Npas4 Antibody (S408-79) [NBP2-59332] - Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S408-79
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Npas4 Antibody (S408-79) - BSA Free Summary

• Description: Novus Biologicals Mouse Npas4 Antibody (S408-79) - BSA Free (NBP2-59332) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Npas4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein amino acids 597-802 (C-terminus) of rat Npas4.
• Localization: Cytoplasm , Nucleus
• Specificity: Detects 90kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPAS4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot 1:1000
• Application Notes: Use in Immunohistochemistry-Frozen was reported in scientific literature (PMID: 31041873).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Npas4 Antibody (S408-79) - BSA Free

• BHLHE79
• bHLHe79neuronal PAS domain-containing protein 4
• Class E basic helix-loop-helix protein 79
• Le-PAS
• neuronal PAS domain protein 4
• Neuronal PAS4
• NXFHLH-PAS transcription factor NXF
• PASD10PAS domain-containing protein 10

Background

Npas4 acts as a transcriptional activator in the presence of ARNT. It can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Npas4 Antibody (NBP2-59332)(1)

Publication using NBP2-59332

• Alharbi M Preoperative anxiety-induced glucocorticoid signaling reduces GABAergic markers in spinal cord and promotes postoperative hyperalgesia by affecting neuronal PAS domain protein 4 Mol Pain 2019-05-02 [PMID: 31041873] (WB, IHC-Fr, Rat)

Bioinformatics

• Gene Symbol: NPAS4