Notch-3 Antibody (603532) [Unconjugated]

BGO1v human embryonic stem cells were stained with Mouse Anti-Human Notch‑3 Monoclonal Antibody (Catalog # MAB1559, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

SUP‑T1 human T cell lymphoblastic lymphoma cell line was stained with Mouse Anti-Human Notch‑3 Monoclonal Antibody (Catalog # MAB1559, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 603532
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Notch-3 Antibody (603532) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Notch-3
  Ala40-Glu467
  Accession # Q9UM47
• Specificity: Detects human Notch-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Notch-3 ICD (aa 2195-2321), rhNotch-1, rhNotch-1 ICD (aa 2251-2556), rhNotch-2, rhNotch-2 ICD (aa 2063-2413), rhNotch-4, rhNotch-4 ICD (aa 1778-2003), recombinant mouse Notch-3, or recombinant rat DLL1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOTCH3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Notch-3 Antibody (603532) [Unconjugated]

• CADASIL
• CASILneurogenic locus notch homolog protein 3
• Notch (Drosophila) homolog 3
• notch 3Notch homolog 3 (Drosophila)
• Notch homolog 3
• Notch3
• Notch-3

Background

Human Notch-3 is a member of the Notch family of type I transmembrane glycoproteins involved in early-event developmental processes. The 2321 amino acid (aa) Notch-3 precursor contains a 1603 aa extracellular region with 34 EGF-like repeats. Repeats 1-11 of human Notch-3 are within the sequence used as an immunogen, and share 94% aa identity with mouse and rat Notch-3. Repeats 11 and 12 are critical for binding the ligands Jagged and Delta. Notch-3 is expressed in vascular smooth muscle, proliferating neuroepithelium, CD4^-CD8^- thymocytes, regulatory T cells and T-ALL leukemia cells. Mutations in the first 5 EGF repeats of Notch-3 in humans can cause CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Notch-3 Antibody (MAB1559)(4)

Publications using MAB1559

• DV Oliveira, KG Coupland, W Shao, S Jin, F Del Gaudio, S Wang, R Fox, JW Rutten, J Sandin, H Zetterberg, J Lundkvist, SA Lesnik Obe, U Lendahl, H Karlström Active immunotherapy reduces NOTCH3 deposition in brain capillaries in a CADASIL mouse model Embo Molecular Medicine, 2022-12-16;0(0):e16556. 2022-12-16 [PMID: 36524456] (ELISA Capture, Mouse)
• H Kim, B Jang, YJ Kim, JC Choi Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects International Journal of Molecular Sciences, 2022-09-11;23(18):. 2022-09-11 [PMID: 36142458] (ELISA Capture, Human)
• Machuca-Parra AI, Bigger-Allen AA, Sanchez AV et al. Therapeutic antibody targeting of Notch3 signaling prevents mural cell loss in CADASIL J. Exp. Med. 7/11/2017 [PMID: 28698285]
• Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi M, Petrucci M, Cippitelli M, Santoni A Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1. J Immunol, 2014-06-09;193(2):950-60. 2014-06-09 [PMID: 24913980] (Flow Cytometry, IHC, Human)

Bioinformatics

• Gene Symbol: NOTCH3
• Uniprot:
  • Q9UM47()