Notch-3 Antibody (2E4D11) - BSA Free

Western Blot: Notch-3 Antibody (2E4D11) [NBP2-52521] - Analysis using Notch3 mAb against human Notch3 (AA: 47-156) recombinant protein. (Expected MW is 37.3 kDa).

Western Blot: Notch-3 Antibody (2E4D11) [NBP2-52521] - Analysis using Notch3 mAb against HEK293 (1) and Notch3 (AA: 47-156)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: Notch-3 Antibody (2E4D11) [NBP2-52521] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, CyTOF-ready
• Clone: 2E4D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Notch-3 Antibody (2E4D11) - BSA Free Summary

• Description: Novus Biologicals Mouse Notch-3 Antibody (2E4D11) - BSA Free (NBP2-52521) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Notch-3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Notch-3 (AA: 47-156) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOTCH3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32169072).
• Theoretical MW: 243.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32169072).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Notch-3 Antibody (2E4D11) - BSA Free

• CADASIL
• CASILneurogenic locus notch homolog protein 3
• Notch (Drosophila) homolog 3
• notch 3Notch homolog 3 (Drosophila)
• Notch homolog 3
• Notch3
• Notch-3

Background

The NOTCH3 gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Notch-3 Antibody (NBP2-52521)(1)

Publication using NBP2-52521

• Fang Ch, Lin Yt, Liang Cm, Liang Sm A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and beta-catenin-ABCG2 signaling J. Biomed. Sci. 2020-03-13 [PMID: 32169072] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: NOTCH3