Notch-2 Antibody (411801) [Alexa Fluor™ Plus 555]

• Reactivity: Hu
• Clone: 411801
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 555

Notch-2 Antibody (411801) [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects human Notch-2 intracellular domain (ICD) in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) Notch-1 ICD, recombinant rat (rr) Notch-1 ECD, rrNotch-2 ECD, rhNotch-3 ECD, or rhNotch‑4 ICD.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Notch-2 Antibody (411801) [Alexa Fluor™ Plus 555]

• AGS2
• hN2
• neurogenic locus notch homolog protein 2
• Notch (Drosophila) homolog 2
• notch 2Notch homolog 2 (Drosophila)
• Notch homolog 2
• Notch2
• Notch-2

Background

Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins.  It is synthesized as a 2446 amino acid (aa) type I TM glycoprotein that undergoes Golgi processing to generate a 180 kDa disulfide-linked extracellular domain (ECD) (likely aa 25-1538) and a 110 kDa membrane bound truncated segment (likely aa 1539-2471).  Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) (aa 1697-2471), a potential nuclear transcription factor.  In the nucleus, NICD regulates CSL pathways.  When NICD is phosphorylated, it is deactivated.  Over aa 2063-2413, human Notch-2 is 94% and 89% aa identical to dog and mouse Notch-2, respectively.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.