NORE1 Antibody - BSA Free

Western Blot: NORE1 Antibody [NB500-248] - Analysis of NORE1 using this antibody at 1:1000 dilution on 293 T cell lysates overexpressed with NORE1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NORE1 Antibody - BSA Free Summary

• Immunogen: Residues 313-329 [DNPQKFALFKRIHKDGQ] of the NORE1 protein. This sequence is located in the RA domain of NORE1.
• Localization: Cytoplasm
• Specificity: This is specific for NORE1, accession number NP_113625.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RASSF5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:150
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500 -1:1000
• Application Notes: This NORE1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Paraffin applications. In WB, a band is seen around 47.1 kDa position.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 47.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NORE1 Overexpression Lysate

Reactivity Notes

Reacts with human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NORE1 Antibody - BSA Free

• Maxp1
• MGC10823
• MGC17344
• New ras effector 1
• NORE1A
• NORE1B
• NORE1RASSF3
• Rap1-binding protein
• RAPLnovel Ras effector 1
• Ras association (RalGDS/AF-6) domain family 5
• Ras association (RalGDS/AF-6) domain family member 5
• ras association domain-containing protein 5
• Ras effector-like protein
• regulator for cell adhesion and polarization enriched in lymphoid tissue
• Regulator for cell adhesion and polarization enriched in lymphoid tissues
• tumor suppressor RASSF3

Background

RASSF1A, a new tumor-suppressor gene located at 3p21.3 is frequently inactivated by promoter region hypermethylation in a variety of human cancers including lung, breast, kidney and neuroblastoma. Another member of the RASSF1 gene family, Ras effector NORE1, which is structurally related to RASSF1, can also mediate a Ras-dependent apoptosis. Moreover, an analysis of NORE1 protein expression showed that it is frequently down-regulated in lung tumor cell lines and primary lung tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NORE1 Antibody (NB500-248)(2)

Publications using NB500-248

• Gezginci-Oktayoglu S, Bolkent S. Ras signaling in NGF reduction and TNF-?-related pancreatic ? cell apoptosis in hyperglycemic rats. Apoptosis;17(1):14-24. 2012-01-01 [PMID: 21938476] (ICC/IF, Rat)
• Miertzschke M et al. Characterisation of interactions of adapter protein RAPL/Nore 1B with Rap GTPases their role in T cell migration. J Biol Chem; 10.1074/jbc.M704361200. [PMID: 17716979]

FAQs for NORE1 Antibody (NB500-248). (Showing 1 - 1 of 1 FAQs).

1. Wwe are interested in your product NB500-248 a NORE1 Antibody, since there are two splice products of the NORE1 gen Nore1A and Nore1B (RAPL) we want to know if your antibody is able to detect both Nore1A and Nore1B. We are basically interested in Nore1B. So my question is, if you can tell me that is recognizing Nore1b or if you have pictures of a Western Blot of cell lysat so that I can take a look if it shows 2 bands with the right size?
   • I am delighted to hear that you are interested in our NORE1 Anitbody. Unfortunately we have not tested whether this recognises both NORE1A and NORE1B. The immunogen is residues 313-329 [DNPQKFALFKRIHKDGQ] of the NORE1 protein. This sequence is located in the RA domain of NORE1, this may be of help to you. I do not have any other image except for the image on the webpage however this is not a good representation of the antibody and it has been provided to us by one of our innovators. I would like reassure you that if the antibody does not work we provide a full refund. You may also be interested in our Innovators Reward Program.

Bioinformatics

• Gene Symbol: RASSF5
• Entrez:
  • 83593(Human)
  • 54354(Mouse)
  • 54355(Rat)
• Uniprot:
  • Q8WWW0(Human)