NONO Antibody (OTI4D9)

Images

 
Western Blot: NONO Antibody (4D9) [NBP2-02060] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: ...read more
Immunocytochemistry/ Immunofluorescence: NONO Antibody (4D9) [NBP2-02060] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO.
Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human prostate tissue using anti-NONO mouse monoclonal antibody.
Flow Cytometry: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NONO antibody, and then analyzed by ...read more
Western Blot: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NONO mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human Ovary tissue using anti-NONO mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI4D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.82 mg/ml

Order Details

NONO Antibody (OTI4D9) Summary

Immunogen
Human recombinant protein fragment correspongding to amino acids 184-385 of human NONO(NP_031389) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NONO
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
54.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NONO Knockout 293T Cell Lysate
Publications
Read Publication using NBP2-02060.

Reactivity Notes

Human, Dog, Rat, Monkey, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.82 mg/ml
Purity
Affinity purified

Alternate Names for NONO Antibody (OTI4D9)

  • NMT5552 kDa subunit
  • non-POU domain containing, octamer-binding
  • NRB54non-POU-domain-containing, octamer-binding
  • p54(nrb)

Background

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NONO Antibody (NBP2-02060)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP2-02060 Applications Species
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NONO Antibody (NBP2-02060) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73034AF350
Alexa Fluor 405 NBP2-73034AF405
Alexa Fluor 488 NBP2-73034AF488
Alexa Fluor 532 NBP2-73034AF532
Alexa Fluor 594 NBP2-73034AF594
Alexa Fluor 647 NBP2-73034AF647
Alexa Fluor 700 NBP2-73034AF700
Alexa Fluor 750 NBP2-73034AF750
Allophycocyanin NBP2-73034APC
Biotin NBP2-73034B
DyLight 350 NBP2-73034UV
DyLight 405 NBP2-73034V
DyLight 488 NBP2-73034G
DyLight 550 NBP2-73034R
DyLight 594 NBP2-73034DL594
DyLight 650 NBP2-73034C
DyLight 680 NBP2-73034FR
DyLight 755 NBP2-73034IR
FITC NBP2-73034F
HRP NBP2-73034H
Janelia Fluor 549 NBP2-73034JF549
Janelia Fluor 646 NBP2-73034JF646
PE NBP2-73034PE
PerCP NBP2-73034PCP

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Bioinformatics

Gene Symbol NONO
Entrez