NONO Antibody (OTI4D9)

Western Blot: NONO Antibody (4D9) [NBP2-02060] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: NONO Antibody (4D9) [NBP2-02060] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO.

Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human prostate tissue using anti-NONO mouse monoclonal antibody.

Flow Cytometry: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NONO antibody, and then analyzed by flow cytometry.

Western Blot: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.

Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NONO mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human Ovary tissue using anti-NONO mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI4D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.82 mg/ml

NONO Antibody (OTI4D9) Summary

• Description: Novus Biologicals Mouse NONO Antibody (OTI4D9) (NBP2-02060) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment correspongding to amino acids 184-385 of human NONO(NP_031389) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NONO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 54.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.82 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NONO Antibody (OTI4D9)

• NMT5552 kDa subunit
• non-POU domain containing, octamer-binding
• NRB54non-POU-domain-containing, octamer-binding
• p54(nrb)

Background

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NONO Antibody (NBP2-02060)(1)

Publication using NBP2-02060

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: NONO
• Entrez:
  • 4841(Human)