non-muscle Myosin IIA [p Ser1943] Antibody (GT144) - Azide and BSA Free

Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA (phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - non-muscle Myosin IIA (phospho Ser1943) antibody [GT144] detects non-muscle Myosin IIA (phospho Ser1943) protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: non-muscle Myosin IIA (phospho Ser1943) protein stained by non-muscle Myosin IIA (phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Human mammary gland. non-muscle Myosin IIA (phospho Ser1943) antibody [144] (NBP3-13579) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA(phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:20000.

Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA(phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:10000.

Immunohistochemistry-Paraffin: non-muscle Myosin IIA [p Ser1943] Antibody (GT144) [NBP3-13579] - Human colon. non-muscle Myosin IIA (phospho Ser1943) antibody [144] (NBP3-13579) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MYH9 (phospho Ser1943) antibody [GT144] detects MYH9 (phospho Ser1943) protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: MYH9 (phospho Ser1943) protein stained by MYH9 (phospho Ser1943) antibody [GT144] (NBP3-13579) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: GT144
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

non-muscle Myosin IIA [p Ser1943] Antibody (GT144) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser1943 of human non-muscle Myosin IIA. The exact sequence is proprietary.
• Modification: p Ser1943
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYH9
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 227 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for non-muscle Myosin IIA [p Ser1943] Antibody (GT144) - Azide and BSA Free

• Cellular myosin heavy chain, type A
• DFNA17
• EPSTS
• FTNSNMMHC-IIA
• MGC104539
• MHANMMHC II-a
• MYH9 variant protein
• MYH9
• Myosin heavy chain 9
• Myosin heavy chain, non-muscle IIa
• myosin, heavy chain 9, non-muscle
• myosin, heavy polypeptide 9, non-muscle
• myosin-9
• NMHC-II-A
• NMMHC II-a
• NMMHCA
• NMMHC-A
• NMMHC-IIA
• Non-muscle myosin heavy chain A
• Non-muscle myosin heavy chain IIa
• nonmuscle myosin heavy chain II-A
• non-muscle myosin heavy polypeptide 9
• Non-muscle Myosin IIA

Background

The non-muscle Myosin IIA gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYH9