NO66 Antibody

Western Blot: NO66 Antibody [NB100-93381] - Staining of A431 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NO66 Antibody Summary

• Description: Novus Biologicals Goat NO66 Antibody (NB100-93381) is a polyclonal antibody validated for use in WB and ELISA. Anti-NO66 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-GAQHSDSKDPR corresponding to internal region according to NP_078920.2.
• Epitope: C-GAQHSDSKDPR
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RIOX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 70 kDa band observed in A431 lysates (calculated MW of 71.1 kDa band according to NP_078920.2). An additional band of unknown identity was also consistently observed at 45 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NO66 Antibody

• chromosome 14 open reading frame 169
• EC 1.14.11.27
• FLJ21802
• hsNO66
• MAPJD
• MYC-associated protein with JmjC domain
• NO66lysine-specific demethylase NO66
• Nucleolar protein 66

Background

NO66 may play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NO66 Antibody (NB100-93381)(1)

Publication using NB100-93381

• Eilbracht J, Reichenzeller M, Hergt M et al. NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. Mol Biol Cell 2004-04-01 [PMID: 14742713]

Bioinformatics

• Gene Symbol: RIOX1
• Entrez:
  • 79697(Human)
  • 71952(Mouse)
• Uniprot:
  • NP_078920.2(Human)