NNT Antibody (1D6)

Images

 
Western Blot: NNT Antibody (1D6) [H00023530-M01] - NNT monoclonal antibody (M01), clone 1D6 Analysis of NNT expression in HepG2.
Immunocytochemistry/ Immunofluorescence: NNT Antibody (1D6) [H00023530-M01] - Analysis of monoclonal antibody to NNT on HepG2 cell. Antibody concentration 10 ug/ml.
ELISA: NNT Antibody (1D6) [H00023530-M01] - Detection limit for recombinant GST tagged NNT is approximately 0.3ng/ml as a capture antibody.
Sandwich ELISA: NNT Antibody (1D6) [H00023530-M01] - Detection limit for recombinant GST tagged NNT is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, S-ELISA
Clone
1D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NNT Antibody (1D6) Summary

Immunogen
NNT (AAH32370, 1 a.a. ~ 207 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK
Specificity
NNT - nicotinamide nucleotide transhydrogenase
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NNT
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Publications
Read Publication using H00023530-M01.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NNT Antibody (1D6)

  • MGC126502
  • mitochondrial
  • nicotinamide nucleotide transhydrogenaseMGC126503
  • Pyridine nucleotide transhydrogenase

Background

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NNT Antibody (H00023530-M01)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NNT
Entrez
OMIM
Uniprot