nNOS Antibody

Images

 
Western Blot: nNOS Antibody [NB120-3511] - Analysis of 40 ug of Mouse (Lane 1) and Rat Brain (Lane 2) tissue lysate.
Immunohistochemistry-Paraffin: nNOS Antibody [NB120-3511] - Normal biopsies of deparaffinized mouse skeletal muscle tissue at dilution 1:400.
Immunohistochemistry: nNOS Antibody [NB120-3511] - Staining of bNOS in rat brain.
Immunohistochemistry-Paraffin: nNOS Antibody [NB120-3511] - Normal biopsies of deparaffinized Rat cerebellum tissue.

Product Details

Summary
Reactivity Mu, Rt, Bv, GP, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

nNOS Antibody Summary

Immunogen
Synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat bNOS.
Localization
Cell Membrane; Sarcolemma; sarcolemmal membrane; peripheral membrane protein. In skeletal muscle, it is localized beneath the sarcolemma of fast twitch muscle fiber by associating with the dystrophin glycoprotein complex.
Specificity
Detects bNOS from bovine, mouse, rabbit and rat tissues. This does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOS1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:8000
  • Immunohistochemistry-Paraffin 1:200 - 400
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Detects an approx. 155 kDa protein representing bNOS from rat brain extract. IHC: Staining of bNOS in rat brain results in staining of the anterior pituitary.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for nNOS Antibody

  • bNOS
  • brain
  • Constitutive NOS
  • EC 1.14.13.39
  • IHPS1
  • Neuronal NOS
  • nitric oxide synthase 1 (neuronal)
  • nNOS
  • N-NOS
  • nNOSNOS type I
  • NOS
  • NOS1
  • Peptidyl-cysteine S-nitrosylase NOS1

Background

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.eNOS and bNOS share ~50% sequence homology, and their activity depends on binding to the calcium/calmodulin complex. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short period of time. bNOS has been found in neurons, peripheral nerve cells, macula densa & pancreatic islet cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NOS1
Entrez