nNOS Antibody (60718) [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF (-)
• Clone: 60718
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

nNOS Antibody (60718) [Alexa Fluor™ Plus 647] Summary

• Immunogen: Partial recombinant human nNOS protein (amino acids 218-1434) [UniProt P29475]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOS1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for nNOS Antibody (60718) [Alexa Fluor™ Plus 647]

• bNOS
• brain
• Constitutive NOS
• EC 1.14.13.39
• IHPS1
• Neuronal NOS
• nitric oxide synthase 1 (neuronal)
• nNOS
• N-NOS
• nNOSNOS type I
• NOS
• NOS1
• Peptidyl-cysteine S-nitrosylase NOS1

Background

NOS (nitric oxide synathase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin (1). Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane and at increased level of Ca2+, nNOS is trasnlocated to cytoplasm. In cytoplasm, nNOS is dephosphorylated by calcineurin which initiates NO production (2). A phosphorylation by PKA and PKC on nNOS leads down-regulation of NO production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOS1