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NMDAR2D Antibody

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ELISA: NMDAR2D Antibody [36340002]
ELISA: NMDAR2D Antibody [36340002]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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NMDAR2D Antibody Summary

Immunogen
This antibody is specific for the C Terminus Region of the target protein.
Specificity
This product is specific for Human GRIN2D.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRIN2D
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • ELISA 1:100-1:2000
Application Notes
This antibody is useful in ELISA and Western Blot.
Control
NMDAR2D Knockout HeLa Cell Lysate
Publications
Read Publications using 36340002.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for NMDAR2D Antibody

  • EB11estrogen receptor binding CpG island
  • glutamate receptor, ionotropic, N-methyl D-aspartate 2D
  • NMDA epsilon 4
  • NMDAR2DN-methyl-d-aspartate receptor subunit 2D
  • N-methyl D-aspartate receptor subtype 2D
  • NR2Dglutamate [NMDA] receptor subunit epsilon-4

Background

NMDAR2D is an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. NMDAR2D is mediated by glycine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: NMDAR2A Antibody [NB300-105] - Immunostaining of rabbit retina showing NR2A in the rod and cone photoreceptors in the outer plexiform layer as well as the entire inner plexiform layer.
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NMDAR1 Antibody
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NB300-118
Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.
Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - The blots show the examples of immunobands against NMDAR1 and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (http://www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - NR1 surface staining of mouse corical neruons. This image was submitted via customer Review.
(14 Reviews)
(16 Publications)
Species: Hu, Mu, Rt
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NMDAR2B Antibody
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Western Blot: NMDAR2B Antibody [NB300-106] - The blots show the examples of immunobands against NMDAR2B (C) and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (http://www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY licence.
Flow Cytometry: NMDAR2B Antibody [NB300-106] - NMDA2B receptor expression on CD4+ T cells. Image supplied by review from confirmed customer.
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Western Blot: NDOR1 Antibody [NBP1-88790] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: NDOR1 Antibody [NBP1-88790] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & intermediate filaments.
Immunohistochemistry-Paraffin: NDOR1 Antibody [NBP1-88790] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Species: Hu, Mu, Rt
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NMDAR2C Antibody
NB300-107
Western Blot: NMDA R, NR2C Subunit Antibody [NB300-107]
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Glutamine
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Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.
Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).
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Western Blot: GluR1 Antibody (S355-1) [NBP2-22399] - Rat brain membrane lysates
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6 Antibody
NBP1-91946
Western Blot: Glutamate Receptor 6 Antibody [NBP1-91946] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
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NBP1-88191
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Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.
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(3 Publications)
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Western Blot: Prion protein Antibody (SC57-05) [NBP2-67569] - Analysis of Prion Protein(PrP) on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence: Prion protein Antibody (SC57-05) [NBP2-67569] - Staining PrP in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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GluR5/GRIK1
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Western Blot: GluR5/GRIK1 Antibody [NBP1-76849] - P815 cell lysate with Glutamate receptor 5 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunocytochemistry/Immunofluorescence: GluR5/GRIK1 Antibody [NBP1-76849] - Staining of Grik1 in mouse brain tissue with Grik1 Antibodyat 20 ug/mL.
Immunohistochemistry: GluR5/GRIK1 Antibody [NBP1-76849] - Staining of Grik1 in mouse brain tissue with Grik1 Antibodyat 5 ug/mL.
Species: Hu, Mu
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GAD1/GAD67 Antibody
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Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161] - Immunocytochemical staining of GAD1/GAD67 (RED/YELLOW) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD1/GAD67 staining was detected using Texas Red goat anti-chicken IgY. MAP2 staining (GREEN) used a rabbit antibody.
Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161] - Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD1/GAD67 (GREEN) immunoreactivity. Phalloidin (F-actin) (RED) staining. DAPI (BLUE) staining.
Immunocytochemistry/Immunofluorescence: GAD1/GAD67 Antibody [NBP1-02161] - Immunohistochemical staining of inhibitory neurons (RED) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The brains were fixed with 4.0% paraformaldehyde. The green staining is low level GFP fluorescence expressed off the actin promoter in this mouse transgenic strain.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
mGluR3 Antibody
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Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS3208] - Anti-GRM3 / MGLUR3 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS3208] - Analysis of anti-GRM3 / MGLUR3 antibody with transfection control.
Immunohistochemistry-Paraffin: mGluR3 Antibody [NLS3208] - Analysis of anti-GRM3 / MGLUR3 antibody with HEK293 human embryonic kidney cells transfected with GRM3 / MGLUR3.
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mGluR7 Antibody
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Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS921] - Anti-GRM7 / MGLUR7 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS921] - Analysis of anti-mGluR7 antibody with human brain, neurons and glia.
Immunohistochemistry-Paraffin: mGluR7 Antibody [NLS921] - Analysis of anti-MGLUR7 antibody with human adrenal.
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Western Blot: Glutamate Receptor 4 Antibody [NBP1-02312] - Staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Glutamate Receptor 4 Antibody [NBP1-02312] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western Blot: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Analysis using GRIK3 mAb against human GRIK3 (AA: extra 32-173) recombinant protein. (Expected MW is 42 kDa)
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Western Blot: GluR7/GRIK3 Antibody (2B3D1) [NBP2-61782] - Analysis using GRIK3 mAb against HEK293 (1) and GRIK3 (AA: extra 32-173)-hIgGFc transfected HEK293 (2) cell lysate.
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Publications for NMDAR2D Antibody (36340002)(2)

We have publications tested in 1 application: MiAr.


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Control Lysate(s)

NMDAR2D Knockout HeLa Cell Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol GRIN2D
Entrez
Uniprot