NMDAR2A Antibody - BSA Free

Western Blot: NMDAR2A Antibody [NBP2-19551] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NMDAR2A antibody (diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody [NBP2-19551] - DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR2A protein stained by NMDAR2A antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: NMDAR2A Antibody [NBP2-19551] - NMDAR2A antibody detects GRIN2A protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. NMDAR2A antibody dilution: 1:500.

Western Blot: NMDAR2A Antibody [NBP2-19551] - Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: NMDAR2A Antibody [NBP2-19551] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NMDAR2A antibody (NBP2-19551) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: NMDAR2A Antibody [NBP2-19551] - NMDAR2A antibody detects NMDAR2A protein at cell membrane by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NMDAR2A stained by NMDAR2A antibody (NBP2-19551) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NMDAR2A Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human NMDAR2A. The exact sequence is proprietary.
• Localization: Cell membrane; Multi-pass membrane protein; Cell junction; synapse; postsynaptic cell membrane
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIN2A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 165 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for NMDAR2A Antibody - BSA Free

• GluN2A
• glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• hNR2A
• NMDA receptor subtype 2A
• N-methyl D-aspartate receptor subtype 2A
• N-methyl-D-aspartate receptor subunit 2A
• subunit epsilon-1

Background

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NMDAR2A Antibody (NBP2-19551)(2)

Publications using NBP2-19551

• Z Du, Y Song, X Chen, W Zhang, G Zhang, H Li, L Chang, Y Wu Knockdown of astrocytic Grin2a aggravates beta-amyloid-induced memory and cognitive deficits through regulating nerve growth factor Aging Cell, 2021-07-22;0(0):e13437. 2021-07-22 [PMID: 34291567]
• Ustunova S, Kilic A, Bulut H et al. Impaired memory by hippocampal oxidative stress in rats exposed to 900 MHz electromagnetic fields is ameliorated by thymoquinone Toxicological; Environmental Chemistry 2022-03-15

Review for NMDAR2A Antibody (NBP2-19551) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Janosch Heller ICC Rat 06/27/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: rat cortical culture (3 weeks old)
• Species: Rat

Comments

• Comments: Fixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT
  
  Blocking Solution & Duration: 3% BSA in PBS with 0.2% saponin, 30 minutes RT
  
  Primary Antibody Diluent and Dilutions Tested: 1:200 in 1% BSA in PBS with 0.2% saponin, over night 4°C
  
  Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-rabbit Alexa647, 1:500 in PBS with 0.2% saponin, 1 hour, RT

Bioinformatics

• Gene Symbol: GRIN2A
• Uniprot:
  • Q12879(Human)