NMDAR2A Antibody (S327A-38) - BSA Free

Western Blot: NMDAR2A Antibody (S327A-38) [NBP2-22404] - Western Blot analysis of Monkey COS cells transfected with GFP-tagged NMDAR2A showing detection of ~170 kDa NMDAR2A protein using Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody, Clone S327A-38 (NBP2-22404). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NMDAR2A. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NMDAR2A Monoclonal Antibody (NBP2-22404) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.

Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody, Clone S327A-38 (NBP2-22404). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody (NBP2-22404) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2A/NMDAR2A Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody, Clone S327A-38 (NBP2-22404). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody (NBP2-22404) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-GluN2A/NMDAR2A Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN2A/NMDAR2A Antibody. (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S327A-38
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NMDAR2A Antibody (S327A-38) - BSA Free Summary

• Description: Novus Biologicals Mouse NMDAR2A Antibody (S327A-38) - BSA Free (NBP2-22404) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A
• Localization: Cell Membrane , Cell Junction
• Specificity: Detects approx 170kDa. Does not react with NR2B.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRIN2A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of GluN2A/NR2A Antibody was sufficient for detection of GluN2A/NR2A in 20 ug of COS cells transiently transfected with GFP-tagged NR2A lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for NMDAR2A Antibody (S327A-38) - BSA Free

• GluN2A
• glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• hNR2A
• NMDA receptor subtype 2A
• N-methyl D-aspartate receptor subtype 2A
• N-methyl-D-aspartate receptor subunit 2A
• subunit epsilon-1

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIN2A
• Uniprot:
  • Q00959(Rat)